Fig. 2 | Nature Communications

Fig. 2

From: Direct imaging of the circular chromosome in a live bacterium

Fig. 2

Domain distribution within the circular chromosome of E. coli. a Example of the automated domain recognition. Top panel, fluorescent image of a toroidal chromosome (deconvolved image). Bottom panel, same image with ridge line (red), equal-intensity lines (thin cyan lines), and blob boundaries (yellow). Scale bar, 2 μm. b Number of blobs per chromosome. Black line shows the probability density function (PDF) of a fitted lognormal distribution. n = 269. c Bar plot of the PDF of the physical size (diameter) of the blobs. d Distribution of domain sizes (DNA length in kbp) in all measured cells. e Blob diameter D (nm) plotted versus the DNA length L (kbp) contained in each blob, on a log-log scale. Circles indicate mean calculated for all cells within a 100 kbp bin size. Red line is a fit of a power law, D ~ Lα with α = 0.60 ± 0.04. Inset shows the same data plotted on linear scales

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