Fig. 7 | Nature Communications

Fig. 7

From: Diverse MR1-restricted T cells in mice and humans

Fig. 7

MR1-restricted TRAV36+ T cell receptors (TCRs) express canonical TCR-α and TCR-β chains. a Table showing CDR3α and CDR3β nucleotide and amino acid sequences from nine TRAV36+ TCRs. Clone names of clones further characterised in this study are written underneath TRAV gene. Germline-encoded residues are high in grey (variable genes = light grey; joining genes = dark grey) and n nucleotides are highlighted in a white box. b Sequence logos showing the amino acid distribution at the CDR3α and CDR3β junction from n = 9 TRAV36+ TCRs. c Flow cytometric histogram overlays showing MR1 (columns 1–4) tetramers, CD1d (column 5) tetramer and CD3 (column 6) staining on HEK293T cells transiently transfected to express human TCRs (left column). Stained cells (transparent histograms) are overlaid on unstained cells (grey histograms). Stained cells are gated for high levels of green fluorescent protein, and mean fluorescent intensity of gated population is depicted in top right of each histogram. Data are representative of two independent experiments

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