Fig. 6 | Nature Communications

Fig. 6

From: Diverse MR1-restricted T cells in mice and humans

Fig. 6

TRAV1-2 MR1 tetramer+ αβ T cells in humans. a Flow cytometric only the first column is colour plots from three representative peripheral blood mononuclear cell (PBMC) samples showing gating strategy of mucosal-associated invariant T (MAIT)-like and non-MAIT-like T cells. In the second column, MAIT cells (black) are overlaid above conventional T cells (grey). In the third column, TRAV1-2 MR1 tetramer+ cells are shown. In the fourth column, histogram overlays show populations as depicted in the key below. b Flow cytometric histogram overlay showing PLZF staining on T cell subsets from one representative PBMC sample. Bar graph showing PLZF expression (normalised to MAIT cells) from a cohort of PBMC samples. c Box and whisker plots showing the proportion of MAIT cells, total TRAV1-2 MR1-5-OP-RU tetramer+ cells, MAIT-like TRAV1-2 MR1-5-OP-RU tetramer+ cells and non-MAIT-like TRAV1-2 MR1-5-OP-RU tetramer+ cells as a percentage of total CD3+ αβ T cells (left), and a percentage of MR1-5-OP-RU tetramer+ αβ T cells (right) with median values depicted above boxes. d Bar graphs showing the co-receptor distribution on T cell subsets from PBMC samples. e Flow cytometric contour plots showing CD218a and CD161 expression on TRAV1-2 MR1-5-OP-RU tetramer+ (left panel) and MR1-6-FP tetramer+ cells (right panel) from two representative donors. Box and whisker plots showing the proportion of MAIT-like cells (CD218a+ CD161+) in TRAV1-2 MR1-5-OP-RU tetramer+ and MR1-6-FP tetramer+ cells (data points on axis = 0). In a, e, n = 18 and in bd, n = 15 donors analysed across 3 independent experiments each. For box and whisker plots, boxes show median, upper and lower quartiles, whiskers represent min. and max. points and ‘+’ represents mean. For bar graphs, error bars represent median ± 95% CI. Source data are provided as a Source Data file

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