Fig. 4 | Nature Communications

Fig. 4

From: Diverse MR1-restricted T cells in mice and humans

Fig. 4

Validation of MR1 reactivity using T cell receptor (TCR) transfectants. Five paired TCRα- and β-chain transcripts from Fig. 3 and one irrelevant CD1d-α-GalCer-restricted TCR were selected for production of transiently transfected surface TCRs on HEK293T cells. Flow cytometric histogram overlays depict staining intensity of these respective transfected HEK293T cells stained with MR1-5-OP-RU, MR1-Ac-6-FP and CD1d-α-GalCer tetramers and are representative of two independent experiments. Cells were pre-gated for high levels of green fluorescent protein and surface TCR, and staining mean fluorescent intensity of gated population (transparent histograms) is depicted in top right of each histogram, overlaid on unstained cells (grey histograms)

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