Fig. 3 | Nature Communications

Fig. 3

From: Diverse MR1-restricted T cells in mice and humans

Fig. 3

Analysis of residual MR1 tetramer+ cells from Traj33 KO mice. a Flow cytometric plots of ex vivo enriched wild-type (WT) and knockout (KO) thymi. Data representative of a combined pool of 3–9 individual mouse thymus per strain. Top row plots were pre-gated on B220 live lymphocytes, second row plots depict combined index sorted single cells and third row plots show the CD4 and CD8 expression from these sorted cells. b Table lists the distribution of Trav/Traj genes out of 111 sequences obtained from sorted MR1-5-OP-RU tetramer+ WT cells and 46 sequences from Traj33 KO cells. c Pie charts illustrate distribution of TRAV genes utilised from WT and KO mice, respectively. Adjacent bar charts show Traj gene usage by TRAV1+ MR1-5-OP-RU tetramer+ cells. d Sequence logos showing the amino acid distribution at the CDR3α junctions from T cell receptors (TCRs) analysed in b, including TRAV1/TRAJ33+ TCRs, TRAV1/TRAJ33 TCRs 12 amino acids in length and TRAV16/TRAJ18+ TCRs 15 amino acids in length. Red rectangle aligns amino acids at position 95 of the respective CDR3α sequences

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