Fig. 1 | Nature Communications

Fig. 1

From: Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response

Fig. 1

Design and surface expression of TRuCs. a Schematic diagram of the lentiviral vector expressing TRuC transgenes. The anti-CD19 scFv is tethered to full-length TCRα, TCRβ, CD3γ, CD3δ, or CD3ε TCR subunits via a flexible glycine serine linker. A T2A peptide enables co-expression of TRuCs and GFP under the EF1α promoter. b The natural TCR complex and five TRuC-containing TCR complexes. c Surface expression of TRuC variants or CARs in primary human T cells. T cells were transduced with lentiviral vectors encoding TRuCs or CARs and expanded in the presence of IL-2. The transduction efficiency was determined by the percentage of GFP-positive cells and the presence of the TRuC on the cell surface by anti-F(ab’)2 staining. d Quantification of the surface expression level of the five TRuCs and two CARs in two independent donors (n = 2). Representative data of two independent experiments are shown

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