Fig. 3 | Nature Communications

Fig. 3

From: Single-molecule kinetics of pore assembly by the membrane attack complex

Fig. 3

AFM shows that initial insertion of C9 is rate limiting in MAC assembly. a Time-lapse AFM imaging of MAC assembly on supported bilayers formed of E. coli lipid extract (see Supplementary Movie 1 for full data set). MAC assembly events initiate at distinct time points, with each MAC pore rapidly completing within a few frames; this implies a rate-limiting-step corresponding to initial insertion of C9. Scale bar: 200 nm; height scale (colour scale bar inset in image for t = 0 min): 40 nm. b Number of MAC assemblies detected (see Methods) with time, indicated as grey squares; the solid red line represents fitting with the function A(1–exp(–t/τinit)), where τinit = 912 ± 32s represents the characteristic time of MAC pore appearance, highlighted by the dashed green lines. The completion time of each individual MAC is much shorter than τinit

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