Fig. 5 | Nature Communications

Fig. 5

From: The cell-wide web coordinates cellular processes by directing site-specific Ca2+ flux across cytoplasmic nanocourses

Fig. 5

Ca2+ flux into nuclear invaginations regulates gene expression. a (from left to right) Confocal z section of Fluo-4 fluorescence in an arterial myocyte (green) ± nuclear label (blue, Draq5), indicating perinuclear cytoplasm (C), nuclear invaginations (I1, I2, I3, I4) and nucleoplasm (N), and fluorescence intensity plot along vertical dashed black line marked in images. b Time series of 3D intensity maps for nuclear region of cell in (a) during application of Angiotensin II (30 μM, white arrow). c (from left to right) 3D reconstruction of section through the nucleus of a myocyte labelled for lamin A (red; confirmed in 54 cells from 14 rats) and showing co-localisation with H3K9me2 (white; confirmed in 14 cells from 5 rats), then same image with digital skin, sectioned and rotated to identify a transnuclear invagination. d, e As in (c) but different cells. fh As in (ce), but showing BAF co-localisation with emerin; confirmed in 10 cells from 4 rats. i, j Dot plots show (mean ± SEM) the effect of blocking RyRs with tetracaine (TTC, 1 mM, 90 min pre-incubation) on MLH1 and S100A9 expression in acutely isolated pulmonary arterial myocytes, assessed by i q-RT-PCR (assayed in triplicate, for n = 3 rats) and j RNAscope (counts per cell, 14–57 cells per plate, n = 9 independent experiments from 3 rats); t-test with Welch’s correction: **p < 0.01. The green and pseudocolour look up tables in (a) and (b) indicate relative fluorescence intensity in arbitrary units

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