Fig. 2 | Nature Communications

Fig. 2

From: Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions

Fig. 2

Structure of the hetero-hexameric LGN:NuMA complex. ab LGN:NuMA hetero-hexamer with LGN molecules depicted with surface representations in red, orange and yellow, and NuMA molecules as coils in different shades of green. Unstructured regions are represented with dotted lines. Panel b highlights the ‘molecular grip’ between the N-terminal LGN-2 capping helix (yellow N-term) with the LGN-1 TPR8 (red C-term). cd Cartoon representations of the hetero-hexameric complex. Panel c is in the same orientation of a, whilst panel d highlights the hooking mechanism of NuMALGNBD N-terminal on the adjacent LGNTPR. An electron density map (Fo-Fc omit map calculated omitting NuMALGNBD chains) is displayed at 2.2-σ contour level. e Excerpt of NuMA sequence alignment encompassing the crystallized fragment, colored by percentage of sequence identity (details in Methods section). Residues built in the crystal structure are indicated with overlying yellow and red lines, those for which there is no electron density with overlying green dotted line. Residues of the NuMA hooking peptide contacting LGN are depicted in bold yellow. f Enlarged view of the region highlighted in the box of panel d, with ball-and-stick representation of the residues involved in the hooking interface between NuMA-1 and LGN-2. A 2Fo-Fc electron density map, contoured at 1-σ level, is displayed

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