Fig. 5 | Nature Communications

Fig. 5

From: High mitogenic stimulation arrests angiogenesis

Fig. 5

Individual ECs with loss of Rbpj/Notch signalling often express p21 and occupy the leading edge. ad The induction of Rbpj genetic deletion from P1 to P3 results in a strong increase of p21 and P-ERK levels at the AF in P6 retinas. Charts show quantification of several large microscopic fields (n = 3 mice per group). Scale bars, 200 μm. e Confocal micrographs of P6 retinas from animals with inducible mosaic deletion of Rbpj in ECs (RbpjiECMosaic-KO) expressing MbTomato-2A-Cre. Images to the right are high-magnification views of boxed areas, showing the indicated signals in more detail. Scale bar, 115 μm. f, g Quantification of mutant (Tomato+) and control (Tomato−) ECs (ERG+) from RbpjiECMosaic-KO retinas (n = 6). Individual cells without Rbpj/Notch signalling (MbTomato-2A-Cre+) are more frequently p21+, especially if they are at the leading edge of retina vessels. In the mature vascular area, most ECs with Rbpj deletion do not upregulate p21. Note that a fraction of Tomato− cells may have Rbpj deletion (compare white bars in f with g). h Cells without Rbpj/Notch signalling (MbTomato-2A-Cre+), are more frequently found at the leading edge of vessels than at the angiogenic front. Error bars indicate std. dev; ***p < 0.0005. Two-tailed unpaired t test. Source data are provided as a Source Data file

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