Fig. 1 | Nature Communications

Fig. 1

From: High mitogenic stimulation arrests angiogenesis

Fig. 1

ECs with low- or high-Notch signalling are outcompeted during vascular development. a, b Confocal micrographs of the postnatal mouse retina vasculature showing that the full deletion of the Rbpj gene from P1 to P6 during retina angiogenesis, results in an increase in endothelial surface and sprouting (isolectinB4) and a decrease in the number of ECs (ERG+) and vascular progression. Cells with deletion of Rbpj from P1 to P3 are usually not found in arterial and peri-arterial endothelium at P6. See details of the iSuRe-Cre allele in Supplementary Fig. 1c–e. Scale bars, 80 μm. ce Comparison of indicated parameters in large microscopic fields of control (n = 5) and mutant (n = 4) mice. f The iChr-Notch-Mosaic and Tie2-Cre mouse lines were crossed to generate fluorescent and genetic mosaics starting at E8.5 in growing ECs. Tissues of mice (n = 4) aged 20 days (P20) were analysed for the presence of Cherry, EGFP or HA-Cerulean cells. Comparison of the P20 ratios to the baseline/initial recombination ratio determined in E9.5 embryos (n = 4) or ES cells (see also Supplementary Fig. 2), shows that ECs with low Notch (EGFP+) or high Notch (HA-Cerulean+) are outcompeted during vascular development. Error bars indicate StDev; **p < 0.005. Two-tailed unpaired t test. Source data are provided as a Source Data file. Scale bars, 50 μm

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