The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

Tian, Weihua; Ye, Zilu; Wang, Shengjun; Schulz, Morten Alder; Van Coillie, Julie; Sun, Lingbo; Chen, Yen-Hsi; Narimatsu, Yoshiki; Hansen, Lars; Kristensen, Claus; Mandel, Ulla; Bennett, Eric Paul; Jabbarzadeh-Tabrizi, Siamak; Schiffmann, Raphael; Shen, Jin-Song; Vakhrushev, Sergey Y.; Clausen, Henrik; Yang, Zhang

doi:10.1038/s41467-019-09809-3

Download PDF

Article
Open access
Published: 30 April 2019

The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

Weihua Tian ORCID: orcid.org/0000-0003-2357-3947¹,
Zilu Ye¹,
Shengjun Wang¹,
Morten Alder Schulz¹,
Julie Van Coillie ORCID: orcid.org/0000-0002-8585-371X¹,
Lingbo Sun¹,
Yen-Hsi Chen¹,
Yoshiki Narimatsu¹,
Lars Hansen¹,
Claus Kristensen²,
Ulla Mandel¹,
Eric Paul Bennett¹,
Siamak Jabbarzadeh-Tabrizi³,
Raphael Schiffmann³,
Jin-Song Shen³,
Sergey Y. Vakhrushev¹,
Henrik Clausen ORCID: orcid.org/0000-0002-0915-5055¹ &
…
Zhang Yang ORCID: orcid.org/0000-0002-4756-5813¹

Nature Communications volume 10, Article number: 1785 (2019) Cite this article

12k Accesses
46 Citations
16 Altmetric
Metrics details

Subjects

Abstract

Lysosomal replacement enzymes are essential therapeutic options for rare congenital lysosomal enzyme deficiencies, but enzymes in clinical use are only partially effective due to short circulatory half-life and inefficient biodistribution. Replacement enzymes are primarily taken up by cell surface glycan receptors, and glycan structures influence uptake, biodistribution, and circulation time. It has not been possible to design and systematically study effects of different glycan features. Here we present a comprehensive gene engineering screen in Chinese hamster ovary cells that enables production of lysosomal enzymes with N-glycans custom designed to affect key glycan features guiding cellular uptake and circulation. We demonstrate distinct circulation time and organ distribution of selected glycoforms of α-galactosidase A in a Fabry disease mouse model, and find that an α2-3 sialylated glycoform designed to eliminate uptake by the mannose 6-phosphate and mannose receptors exhibits improved circulation time and targeting to hard-to-reach organs such as heart. The developed design matrix and engineered CHO cell lines enables systematic studies towards improving enzyme replacement therapeutics.

Multiplexed engineering glycosyltransferase genes in CHO cells via targeted integration for producing antibodies with diverse complex-type N-glycans

Article Open access 21 June 2021

Ngan T. B. Nguyen, Jianer Lin, … Yuansheng Yang

Optimizing human α-galactosidase for treatment of Fabry disease

Article Open access 23 March 2023

William C. Hallows, Kristen Skvorak, … Gjalt W. Huisman

Small-molecule control of antibody N-glycosylation in engineered mammalian cells

Article 20 May 2019

Michelle M. Chang, Leonid Gaidukov, … Ron Weiss

Introduction

Lysosomal storage diseases (LSDs) are characterized by the progressive accumulation of undegraded metabolites that lead to lysosomal and cellular dysfunction^1,2. A variety of therapeutic approaches have been developed for LSDs, with intravenous enzyme replacement therapies (ERTs) being the most prevalent³, but ERTs for LSDs still face major challenges, and the most important may be the delivery of the infused recombinant enzymes to hard-to-reach organs, such as bone, kidney, heart, and brain⁴. Lysosomal enzymes are glycoproteins and the cellular uptake of replacement enzymes is thought to primarily rely on cell-surface receptors recognizing N-glycan features⁵, including the mannose 6-phosphate (M6P) receptors (MPRs)⁶, Ashwell–Morell receptor (AMR) (asialoglycoprotein receptor)⁷, and mannose receptor (MR)⁸, and these participate in cellular uptake and lysosomal delivery of therapeutic glycoproteins. The MPRs specifically recognize terminal M6P attached to high-mannose and hybrid-type N-glycans, and they direct both intracellular delivery of lysosomal enzymes as well as uptake of exogenous M6P-containing glycoproteins from circulation⁶. The AMR expressed primarily on liver hepatocytes recognizes glycoproteins with uncapped terminal galactose (Gal) or N-acetyl-galactosamine (GalNAc) residues, and mediates clearance from the circulation⁷. The MR expressed primarily on mononuclear macrophages binds mainly exposed mannose (Man), N-acetyl-glucosamine (GlcNAc) and fucose (Fuc) residues on N-glycans, and directs uptake of glycoproteins and targeting to endosomes and lysosomes⁸. Tissue distribution and circulation time of infused replacement enzymes are at least partly dependent on the expression of these receptors⁹. Other glycan-binding proteins including Siglecs and Galectins may bind therapeutic N-glycoproteins^10,11, and glycan-independent uptake of lysosomal enzymes by, for example, the low-density lipoprotein receptor proteins (LRPs) has been reported¹². The glycosylation state of replacement enzymes is critical for the pharmacokinetic properties and therapeutic effect, with the key determining N-glycan features being the degree of M6P tagging and exposure of terminal Man, Gal, and/or GlcNAc residues in a complex interplay yet unexplored. Most currently approved ERTs have highly heterogeneous N-glycan structures as dictated by the inherent glycosylation capacity of CHO cells¹³. However, there have been limited options for custom design of the glycosylation capacity of CHO cells and thus for testing specific ERTs with different N-glycan features to explore potential improved therapeutic performance.

Different strategies have been undertaken to explore glycoengineering as a means to improve delivery of ERTs, including use of exoglycosidases for postproduction enzyme modification¹⁴, as well as the use of engineered yeast¹⁵ and plant production platforms^16,17. Pioneering work originally demonstrated how glycosidase trimming of N-glycans on β-glucoscerebrosidase (GBA) resulted in efficient targeting to macrophages through the MR and provided a successful therapy for non-neuropathic Gaucher disease¹⁸. The first recombinant GBA with Man-terminated (high-Man) N-glycans was produced in CHO cells followed by postproduction exoglycosidase treatment¹⁹, and similar GBA products are produced in human cells by use of mannosidase I inhibitor (kifunensine)²⁰ or in carrot cells²¹. Most strategies for glycoengineering of lysosomal enzymes have sought to improve targeting by MPRs and MRs by increasing the content of M6P or exposed Man residues^15,22,23,24. However, these glycoforms will cause rapid and efficient uptake by especially the liver and spleen, while targeting to other organs may be limited⁴. Early studies demonstrated that increased content of sialic acid (SA) on lysosomal enzymes isolated from plasma improves their circulation time similar to other types of therapeutic glycoproteins²⁵, but further studies were hampered by lack of methods to produce these recombinantly. Oxidative degradation and reduction of glycans on enzymes has provided therapeutic efficacy with extended circulation time and wider biodistribution²⁶; however, this partly inactivates the enzyme and may not be suitable for clinical production^27,28.

Most ERTs are produced in CHO cells, and with the advent of efficient precise gene editing tools, it is now possible to introduce extensive engineering designs to optimize the glycosylation capacity of CHO cells²⁹. Here, we present a comprehensive screen of engineering options for lysosomal enzymes in CHO cells, and we provide a panel of glycoengineered CHO cell lines with different capacities for producing lysosomal enzymes furnished with all the key glycan features known to affect cellular uptake and circulation time. The genetic design matrix developed makes it possible to investigate ERTs with a diverse array of glycoforms. We used the α-galactosidase A (GLA) as a representative ERT in a mouse model of Fabry disease, and demonstrate how distinct glycoforms of GLA are differentially targeted to liver, spleen, kidney, and heart, and present evidence that GLA glycoforms capped with α2-3-linked SA (α2-3SA), but surprisingly not α2-6SA, exhibit improved circulation and biodistribution. Thus, in contrast to the current dogma, α2-3SA-capped glycoforms of lysosomal enzymes may represent a strategy to overcome the most critical problems of rapid clearance in liver and poor biodistribution found with current ERTs.

Results

Glycoengineering of lysosomal enzymes produced in CHO cells

A stable wild-type (WT) CHO clone expressing human GLA was established and used for a gene knockout (KO) targeting screen using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) considering all glycosyltransferases and hydrolases functioning in N-glycosylation and M6P processing, as well as receptors and other proteins (Fig. 1). RNA-sequencing expression profiling was used to identify relevant genes expressed in CHO cells (Supplementary Fig. 1). We used site-specific glycoprofiling of the secreted purified GLA to monitor effects on glycosylation. The occupancy at individual N-glycosites was evaluated by comparing the peptide/glycopeptide ratio for each N-glycosite. GLA has three N-glycosites (N108, N161, and N184), and when expressed in CHO WT cells, GLA was site-specifically glycosylated with mainly complex structures capped with SA at N108 and with M6P-tagged high-mannose-type glycans at N161 and N184 (Fig. 2a and Supplementary Fig. 2, #1–3), in agreement with previous reports³⁰. We targeted 43 genes individually or in rational combinations guided by the sequential biosynthetic pathway of N-glycans and known groups of isoenzymes with overlapping functions (Supplementary Table 2 and Supplementary Data 1). Figure 1 presents a summary of the observed general trend effects of the screen for SA, M6P, and Man. In general the occupancies at the three glycosites were found to be near complete for GLA produced in the CHO mutants, with the exception of the N184 glycosite where KO of Alg5 and Alg6 reduced the occupancy.

Targeting the lipid-linked oligosaccharide precursor assembly on the cytosolic side (Alg1/2/11/13/14) was not successful since viable cells with bi-allelic KO could not be established in agreement with similar observations in yeast^31,32; however, targeting the precursor assembly on the ER luminal side (Alg3/5/6/8/9/12) produced surprising options for site-specific engineering of M6P-tagging of GLA. KO of Alg3 substantially enhanced M6P tagging at N108, while reducing M6P at N161 (Fig. 2b and Supplementary Fig. 2, #4–5). KO of Alg9 reduced M6P at N161 and increased tagging at N184 (Fig. 2c and Supplementary Fig. 2, #6). KO of Alg12 reduced M6P at N161 and increased M6P at N184 (Fig. 2d and Supplementary Fig. 2, #7). KO of Alg6 and Alg8 enhanced hybrid structures with one branch capped by SA and one with M6P at N161 (Fig. 2e, f and Supplementary Fig. 2, #9–10). KO of cis-Golgi mannosidases (Man1a1/1a2/1b1/1c1) enriched oligomannose structures and enhanced M6P at all three glycosites (Fig. 2g and Supplementary Fig. 2, #12–16). KO of medial Golgi mannosidase (Man2a1/2) created hybrid N-glycans with one branch capped by SA and one with oligomannose at the expense of reduced M6P (Fig. 2h and Supplementary Fig. 2, #17). KO of Mgat1 as expected completely eliminated complex N-glycans, and interestingly enhanced M6P tagging at N161 and N184 (Fig. 2i and Supplementary Fig. 2, #18). KO of Mgat2 produced the mono-antennary hybrid-type N-glycan at N108 without affecting M6P at N161 and N184 (Fig. 2j and Supplementary Fig. 2, #19), while KO of Mgat4b/5 completely eliminated tri- and tetra-antennary N-glycans and increased homogeneity (Fig. 2k and Supplementary Fig. 2, #20). The results demonstrate how the content and position of M6P and exposed Man on lysosomal enzymes can be fine-tuned in great detail by gene engineering of CHO cells. Targeting the N-glycan ER glucosidases (Mogs/Ganab) to probe the role of the Glc residues and chaperone interactions did not affect secretion of GLA substantially (Supplementary Fig. 3), and demonstrated that GLA glycoforms with retained Glc residues and M6P tagging can be produced (Fig. 2l, m and Supplementary Fig. 2, #21–22).

Targeting the M6P tagging process by KO of Gnptab or Gnptg of the GlcNAc-1-phosphotransferase complex enabled production of GLA with rather homogeneous complex N-glycans capped by SA at all N-glycosites, but lacking M6P residues (Fig. 2n, o and Supplementary Fig. 2, #23–24). In addition, KO of the GlcNAc-1-phosphate hydrolase (Nagpa) uncovering enzyme resulted in GLA with GlcNAc residues retained on M6P and interestingly increased M6P tagging, including substantial increase in bis-M6P (Fig. 2p and Supplementary Fig. 2, #26). In addition to two high-affinity M6P binding sites, the large cation-independent mannose 6-phosphate receptor (CI-MPR) contains another preferential binding site for M6P-GlcNAc³³. Targeting the M6P-tagging process may also affect lysosomal targeting of some endogenous CHO cell lysosomal enzymes^12,34, and resulting changes in secreted lysosomal glycosylhydrolases, for example, neuraminidase 1 (Neu1) may affect glycan structures of recombinant expressed enzymes. KO of B4galt1/3 reduced galactosylation and resulted in exposed GlcNAc residues primarily at N108 (Fig. 2q and Supplementary Fig. 2, #31). Targeting sialylation by KO of St3gal4/6 substantially reduced SA capping and resulted in the exposure of terminal Gal residues (Fig. 2r and Supplementary Fig. 2, #32). Furthermore, KO of Fut8 eliminated core fucose without affecting other features (Fig. 2s and Supplementary Fig. 2, #33).

We also targeted the genes encoding the M6P receptors CI-MPR (Igf2r) and CD-MPR (M6pr), which did not substantially affect glycosylation of the secreted GLA, although KO of Igf2r slightly increased bis-M6P tagging at the N184 glycosite (Supplementary Fig. 2, #34–35). Targeting the late-acting signal peptidase, Sppl3, shown to play a role in shedding of glycosyltransferases³⁵, induced a slight increase of exposed Man (Supplementary Fig. 2, #38). KO of Furin, important for activation of Nagpa³⁶, resulted in similar N-glycan profile with the accumulation of GlcNAc-1-P residues, as found with KO of Nagpa (Fig. 2t and Supplementary Fig. 2, #39). KO of phosphokinase Fam20c and the phosphatidylinositol-4-phosphate effector Golph3 and Golgi protein Golph3l did not substantially affect the N-glycosylation of GLA (Supplementary Fig. 2, #40–42).

Combinatorial glycoengineering

The individual gene KO screen provides a matrix for design of combinatorial engineering to produce GLA with a wider range of desirable glycoforms. We first explored designs without M6P tagging. Stacking KO of Gnptab/g with KO of Mgat1 enabled production of GLA with high-mannose N-glycans at all three glycosites (Fig. 3a and Supplementary Fig. 2, #44), which will bind MR expressed on macrophages and efficiently target the liver and spleen^{19,37,38,39,40}. Stacking KO of Man2a1/2 involved in α-mannosidase trimming generated GLA with a mono-antennary hybrid structure with a complex sialylated α3-arm combined with three Man residues on the α6-arm (Fig. 3b and Supplementary Fig. 2, #45). Stacking KO of Mgat2 enables production of GLA with mono-antennary hybrid N-glycan (Fig. 3c and Supplementary Fig. 2, #46), and stacking KO of Mgat4b/5 enables production of GLA with homogeneous bi-antennary complex N-glycans with SA capping (Fig. 3d and Supplementary Fig. 2, #47), which could be combined with Mgat2 KO²⁹.

Next, we focused on improving M6P tagging by first testing individual knock-in (KI) of GNPTG or GNPTAB, which enhanced M6P at N161 and N184, and KI of GNPTAB induced bis-M6P at N184 (Fig. 3e, f and Supplementary Fig. 2, #48–51). Moreover, combined KI of both genes induced a substantial increase in M6P tagging at all three glycosites and with high content of the mono-antennary hybrid structure with SA and M6P (Fig. 3g and Supplementary Fig. 2, #52–53). KI of GNPTAB combined with KO of Alg3 enabled production of a unique high-Man N-glycan with efficient M6P tagging at all N-glycosites (Fig. 3h and Supplementary Fig. 2, #54-55). The CI-MPR has multiple binding sites and has the capacity to bind diverse M6P-tagged structures with different affinities⁴¹, and increasing the M6P content and introducing bis-M6P are predicted to enhance uptake as demonstrated, for example, with the acid α-glucosidase used for ERT of Pompe disease²³.

CHO WT cells only have capacity for α2-3SA capping, and systematic studies of the influence of α2-3SA versus α2-6SA capping found on most human serum glycoproteins have not been performed with native glycoproteins. Targeted KI of ST6GAL1 in cells with KO of Gnptab and St3gal4/6 enabled production of GLA with homogeneous α2-6SA capping (Fig. 3i and Supplementary Fig. 2, #58). Combining KO of Mgat4b/5 with KI of ST3GAL4 enabled production of homogenous bi-antennary N-glycans capped with α2-3SA (Fig. 3j and Supplementary Fig. 2, #59). Combined with KO of Fut8, any glycoform may likely be produced without core fucose (Fig. 3k, l and Supplementary Fig. 2, #60, 61).

The glycoengineering matrix is applicable to other ERTs

To validate the glycoengineering designs for other lysosomal enzymes, we tested representative designs with GBA that has four N-glycans (Fig. 4 and Supplementary Fig. 4 and Supplementary Tables 3 and 4)³⁸. KO of Alg3 increased the M6P content of GBA in particular for N146 and N270 glycans (Fig. 4b and Supplementary Fig. 4, #2). KO of Alg9 had little effect on N-glycans at N19 and N59, but altered the oligomannose structures with M6P at N146 and N270 (Fig. 4c and Supplementary Fig. 4, #3). Targeting Gnptab resulted in rather homogeneous complex type N-glycans with SA capping at all four N-glycosites and no M6P content (Fig. 4d and Supplementary Fig. 4, #4). Targeting Mgat1 enabled production of GBA without complex type N-glycans, but with high-mannose glycans and reduced M6P mainly at N270 (Fig. 4e and Supplementary Fig. 4, #5–6). Stacked KO of Gnptab and Mgat1 enabled production of GBA with high-mannose N-glycans (4–5 Man) without M6P at all glycosites (Fig. 4f and Supplementary Fig. 4, #7–8). Stacked KO of Man2a1/2 and Gnptab generated GBA with a rather homogeneous mono-antennary hybrid structure at all four glycosites with a complex sialylated α3-arm combined with three Man residues on the α6-arm (Fig. 4g and Supplementary Fig. 4, #9). Similarly, GBA with mono-antennary hybrid N-glycans carrying a single Man residue at the α6-arm was generated by stacking KO of Mgat2 and Gnptab (Fig. 4h and Supplementary Fig. 4, #10). In general, the outcome of the engineering performed with GBA correlated well with the effects observed with GLA, when considering the inherent site specificity of N-glycan processing found with the enzymes expressed in WT CHO cells.

Analyses of GLA glycoforms in a Fabry mouse model

Fabry disease is caused by deficiency in GLA activity, and the leading ERT is Fabrazyme (Sanofi Genzyme) produced in CHO cells³⁰. We first benchmarked GLA produced in our CHO WT cell (~100 mg/L) with a clinical lot of Fabrazyme finding lower content of exposed Man residues on GLA produced in our CHO WT cells (Fig. 5a and Supplementary Fig. 2, #1, 3). The GLA enzyme produced in our CHO WT cells exhibited similar blood circulation half-time (12.0 ± 0.3 min) (mean ± standard deviation) as Fabrazyme (11.9 ± 2.3 min) (Fig. 5b), but with trends of higher liver targeting and lower spleen, kidney, and heart targeting of our GLA variant compared to Fabrazyme (4 h after infusion), although only the lower kidney targeting was significant (Fig. 5d). We chose to use Fabrazyme for comparison in further studies to enable direct comparison with results from the literature.

We then tested five distinct glycoforms of GLA (Fig. 5a and Supplementary Fig. 2, #6, 55, 52, 59, 58). The specific activity and stability in plasma of these GLA glycovariants were essentially identical (Supplementary Fig. 6). In a first experiment we used one infusion dose of 1 mg/kg (Exp. #1) (Fig. 5c, e). The three glycoforms designed with slightly lower M6P (GLA-LoM6P), higher M6P (GLA-HiM6P), or higher M6P content with mainly the hybrid type (GLA-HybM6P) produced trends towards higher or lower circulation time with half-lives of 15.4 ± 1.1, 11.0 ± 2.0, and 8.3 ± 0.8 min, respectively, compared with 9.8 ± 0.3 min for Fabrazyme (Fig. 5c). These three glycoforms showed only minor differences in targeting to select organs compared to Fabrazyme (24 h after infusion), with the LoM6P glycoform yielding significantly higher levels of enzyme activity in the heart, and the HiM6P and HybM6P glycoforms exhibiting lower levels in the spleen (Fig. 5e).

In striking contrast, the two glycoforms designed with N-glycans capped by SA and without M6P and exposed Man produced significant changes in circulation and biodistribution (Fig. 5c, e, f). GLA-Bi23SA with homogeneous bi-antennary N-glycans capped with α2-3SA (Fig. 5a) exhibited a markedly extended (3-fold) circulation time (half-life 27.5 ± 0.8 min) (Fig. 5c) and lower enzyme activity in the liver, spleen, and kidney, but the highest level of enzyme in the heart among all glycoforms tested (90% increase) (Fig. 5e). Importantly, the GLA-LoM6P showed the same trend as would be predicted. The most frequent cause of death in patients with Fabry disease is cardiomyopathy, and increased delivery to the cardiovascular system with glycoforms such as GLA-Bi23SA may present a promising solution. The circulation time of GLA is partly affected by low stability of the enzyme in the plasma at neutral pH. A number of contemporary studies have reported that GLA has poor stability in unbuffered human plasma in vitro with a loss of more than 50% activity within 15 min^16,42; however, originally Desnick et al.²⁵ demonstrated better stability of GLA in neutral-buffered human plasma with a half-life over 200 min. We therefore tested the stability of all GLA glycovariants in mouse plasma with and without buffering with HEPES, and confirmed extended stability with 50% loss of activity at 3 h for Fabrazyme and all GLA variants with buffering (Supplementary Fig. 6). To further analyze the in vivo clearance of GLA variants, we tested mouse plasma by Western blotting (Supplementary Fig. 7), and found that loss of an immunoreactive GLA band migrating at 52 kDa correlated well with loss of enzyme activity and especially slower clearance of GLA-Bi23SA (Fig. 5c).

GLA-26SA with N-glycans capped by α2-6SA demonstrated only marginally elevated circulation time (Fig. 5c), and perhaps surprisingly⁴³, resulted in higher liver uptake and corresponding decrease in the spleen and kidney uptake (Fig. 5e). The striking increase in liver uptake resembles previous results obtained with albumin neoglyconjugates suggesting interaction of NeuAcα2-6Galβ1-4GlcNAc terminating glycans with the AMR⁴⁴, although other studies suggest that primarily NeuAcα2-6GalNAcβ1-4GlcNAc-terminated and -non-sialylated neoglycoproteins are removed from circulation⁴³. AMR-mediated uptake of α2-6SA-capped ERTs is predicted to be considerable less efficient compared to the asialo-glycoform based on previous studies demonstrating circulatory half-life of about 1 min for desialylated glucocerebrosidase compared to 21 min for the native enzyme⁴⁵.

Cellular localization of Fabrazyme and the glycovariants in the heart, kidney, and liver was assessed by immunohistochemistry (IHC) (Fig. 5f). The localization pattern of Fabrazyme in these organs was consistent with that of agalsidase alfa reported in previous studies^17,46. In the heart, Fabrazyme and all five glycovariants were detected in vascular and/or perivascular cells, but not in cardiomyocytes (Fig. 5f). There were no clear differences between the tested variants. In the kidney, Fabrazyme and GLA-LoM6P, GLA-HiM6P, GLA-HybM6P, and GLA-Bi23SA were predominantly detected in tubular epithelial cells. However, GLA-26SA had significantly decreased number and intensity of positive signals in tubules compared to the other variants tested (Fig. 5f). In the liver, Fabrazyme, GLA-LoM6P, GLA-HiM6P, and GLA-HybM6P were detected in hepatocytes, putative Kupffer cells, and endothelial cells of sinusoidal capillaries. GLA-Bi23SA was also detected in these cell types; however, the number of positive signals in hepatocytes was clearly decreased compared to Fabrazyme. Distribution of GLA-26SA in the liver was remarkably different from other variants; this variant was detected almost exclusively in hepatocytes, and the number of positive signals in hepatocytes was clearly increased compared to Fabrazyme (Fig. 5f).

Encouraged by the improved properties of GLA-Bi23SA with extended circulatory half-life, and GLA-26SA with altered biodistribution, we proceeded to test reduction of accumulated globotriosylceramide (Gb3) substrate in organs 2 weeks after a single injection of 1 mg/kg. We performed two independent series with a single 1 mg/kg dose (Exp. #1) of Fabrazyme and GLA-Bi23SA, and subsequently GLA-26SA (Fig. 5g). GLA-Bi23SA produced the same reduction of the Gb3 content in the heart, kidney, and liver as compared to Fabrazyme (Fig. 5g), and this corresponds to the greatest reduction reported so far with any enzyme strategy used in the Fabry mouse model^16,17,47. GLA-26SA produced lower reduction of Gb3 levels in the heart and kidney compared to Fabrazyme and GLA-Bi23SA, while the effect in the liver was similar for all variants. This finding correlates with the lower level of GLA-26SA distributed to the heart and especially kidney (Fig. 5e). We did not observe complete reduction of Gb3 levels in the kidney and heart in agreement with previous reports^17,47, and the reason for the incomplete reduction is unclear and may be due to multiple factors, including that Gb3 and the Globo-series glycolipids are particularly abundant in the kidney^17,47. Importantly, previous studies using higher dose or repeated infusion of Fabrazyme or other GLA variants have not resulted in further complete reduction of Gb3 in the heart and especially in the kidney⁴⁷.

To further explore the performance of GLA-Bi23SA, we tested two lower doses (0.5 and 0.2 mg/kg) in comparison with Fabrazyme (Expt. #2), where we analyzed enzyme activity in organs after 1 week instead of 24 h (Fig. 5e) and residual Gb3 after 1 week instead of 2 weeks (Fig. 5g). This confirmed the improved distribution of GLA-Bi23SA to the heart and lower uptake in the liver compared to Fabrazyme, but also demonstrated dose dependency, suggesting that further studies of optimal dosing for GLA-Bi23SA is needed. More importantly, this clearly demonstrated detectable levels of enzyme activity 1 week after infusion and thus providing strong evidence in support of lysosomal delivery of GLA-Bi23SA in agreement with the immunohistochemistry results (Fig. 5f). The analysis of Gb3 levels in the heart and kidney confirmed the efficient function of both GLA-Bi23SA and Fabrazyme, but also showed that further studies of dosing are needed. These results unequivocally demonstrate that glycoforms of GLA without the classical receptor ligands M6P and Man are efficiently taken up by cells, delivered to the lysosome, and function in reduction of Gb3.

In summary, the glycoengineered GLA variants exhibited distinct pharmacodynamic profiles in Fabry mice. The α2-3SA sialylated design without M6P and terminal Man led to reduced uptake in the liver, prolonged plasma half-life, and improved delivery to the heart. In contrast, the α2-6SA sialylated design led to preferential delivery to hepatocytes and decreased uptake by renal tubular cells. The longer circulation time of GLA-Bi23SA is likely to provide opportunity for wider organ distribution as evidenced by the marked increase in uptake in the heart, and may also provide opportunity for lower dose or less frequent dosing of replacement enzymes, although further studies are needed to address this.

Discussion

The comprehensive engineering performed with GLA and GBA in CHO cells demonstrates ample options for fine-tuning all key features of N-glycans on lysosomal enzymes known to be important for their cellular uptake, circulation time, and biodistribution. This includes a high degree of site-specific fine-tuning of M6P stoichiometry, exposure of Man, Gal, and GlcNAc residues, and capping by SA. We provide novel designs for recombinant lysosomal enzymes that lack recognition markers for classical MPRs and MRs, but contain homogenous N-glycans capped by SA. Among these we discovered that the GLA-Bi23SA design offers increased circulation time, efficient cellular uptake, and improved organ distribution in a Fabry mouse model despite the lack of M6P and exposed Man residues. Although these preliminary studies did not demonstrate improved substrate reduction in organs, the results suggest that the α2-3SA design potentially may be used to overcome one of the arguably major obstacle for many ERTs, that is, their rapid clearance from circulation by the liver and spleen. Extended circulation is predicted to enable wider biodistribution and possibly transport across the blood–brain barrier⁴⁸. The achieved control of N-glycosylation in CHO cells meets or surpass the glycoengineering opportunities previously presented with non-mammalian cells and postproduction modification strategies^{14,15,16,17,18,19,22,23,24,40}. The clinical features of LSDs and the organs affected differ greatly as do the biostructural properties of the respective deficient enzymes, and the design matrix and glycoengineered CHO cells developed here will be valuable tools for production and testing of optimal designs for individual ERTs, in order to improve a class of essential drugs with high costs and poor performance.

CHO cells are the preferred mammalian expression hosts for human therapeutics. Given the recent options for targeted and stable gene engineering of glycosylation capacities in mammalian cells²⁹, we undertook to explore the glycoengineering options for M6P-modified lysosomal replacement enzymes that represent one of the most complex challenges for the biopharmaceutical industry^3,49,50. Using GLA as an illustrative example, we dissected virtually all steps in the genetic and biosynthetic control of N-glycosylation and M6P tagging, and found surprising plasticity and control for fine-tuning complex N-glycan patterns even with a degree of glycosite specificity (Figs. 2 and 3). Thus, M6P tagging could be tuned up and down and directed to one (N184), two, or all three N-glycosites of GLA, and importantly combined with different degrees of high-Man or complex sialylated N-glycans. Moreover, we also demonstrated production of glycoforms with homogenous SA capping, but lacking M6P or exposed Man residues.

It has long been clear that the structure of N-glycans on replacement enzymes affects cellular uptake and circulation time by interacting with different cell-surface receptors⁵¹, and that altering the glycan composition can be used to direct organ targeting. This was demonstrated first with targeting of GBA with high-Man structures for the MR on macrophages⁵², and ERTs with glycans optimized for targeting specific receptors are already successfully used in the clinic^18,37. Different strategies have been applied to optimize N-glycans for specific cell and organ targeting requirements. To achieve N-glycans with high degree of Man exposure for MR-mediated liver targeting, for example, for GBA treatment of Gaucher patients, the industry has used plant cells⁴⁰, human fibrosarcoma cells combined with N-glycan mannosidase inhibitors³⁸, and CHO cells combined with postproduction treatment with multiple exoglycosidases⁵³. We present engineered CHO cells capable of producing this high-Man glycoform of GBA (Fig. 4f), and importantly also related designs with different degrees of Man exposure and SA capping expected to influence kinetics of uptake and circulation half-life (Fig. 4g). To increase the M6P content in particular for targeting muscle cells, yeast has been used to produce the lysosomal α-glucosidase deficient in Pompe disease¹⁵. Yeast modify human lysosomal enzymes with Man-Pi-6-Man, but the elegant introduction of an uncovering α-mannosidase enzyme results in the production of α-glucosidase rich in M6P¹⁵. Other strategies to increase M6P content include in vitro chemical conjugation^22,24, or co-expression of a truncated GlcNAc-1-phosphotransferase α/β precursor⁵⁴. These strategies do not enable fine control over the content (or site specificity) of M6P and other glycan features including SA capping, and the presented engineering of high-M6P glycoforms in CHO cells fully match these strategies (Fig. 3f, g, h). Other postproduction modification strategies including oxidative reduction of glycans²⁶ and PEGylation¹⁶ have been applied to reduce glycan-mediated receptor uptake and/or enhance circulation, and these may be met by the presented glycoform designs with homogeneous SA capping, but lacking M6P or exposed Man residues (Fig. 3j). Thus, our study suggests that any of the more complex processes used for production of enzymes required for ERTs in the clinic today or in development^{14,15,16,17,18,19,22,23,24,40} can be produced simpler and more effective in glycoengineered CHO cells. Moreover, there may be advantages in combining distinct glycoforms of lysosomal enzymes with emerging glycosylation-independent targeting strategies developed for blood–brain barrier transport^37,55,56.

Our understanding of and ability to predict the outcome of interactions between glycoproteins with heterogeneous N-glycans presenting these features and the multiple receptors involved is limited. Numerous studies have explored the binding and uptake of extreme glycoforms, such as high-Man- and high/low-M6P-containing lysosomal enzymes^6,7,8,9, but systematic studies investigating the complex interplay between different glycan features have not been possible due to the lack of methods to produce such glycoforms. Studies with, for example, the lysosomal α‐mannosidase that contains multiple N-glycans with very low M6P content and exposure of Man when produced in WT CHO cells suggest that limited interaction with the MPRs and MR may be advantageous for wider biodistribution and crossing into the brain, possibly due to extended circulation time^48,57. Similar findings were observed with postproduction modified enzymes with partially destroyed glycans²⁶. Here, we explored five distinct glycoforms of GLA, including two lacking M6P or exposed Man residues in a Fabry disease mouse model, and found significant changes in circulation half-life and biodistribution (Fig. 5). Most significantly, the GLA glycoform with α2-3SA-capped N-glycans not only showed enhanced circulation time but also demonstrated efficient uptake and function in all tested organs with improved distribution to the hard-to-reach heart compared to the leading Fabrazyme variant (Fig. 5e, f). Evaluating the relative organ distributions of glycovariants among the four major visceral organs tested illustrate the substantial improved distribution of GLA-Bi23SA to the heart and other organs except the liver (Supplementary Fig. 8). The mechanism for uptake of the α2-3SA-capped GLA glycoform is not clear, but studies have shown that lysosomal targeting of GLA is not exclusively dependent on M6P tagging^12,58, and endocytic receptors including sortilin (SORT1) and megalin (LRP2) that do not bind glycan features have been shown to serve in the uptake of GLA^59,60. The 3-fold increase in circulatory half-life for α2-3SA-capped GLA is lower than the increase observed with, for example, oxidative degradation and reduction of the β-glucuronidase²⁶, but this likely reflects the lower stability of GLA in the plasma^16,25,42. It may be interesting to explore combining this glycoform with the stabilizing molecular chaperone AT1001^61,62 or pegylation¹⁶, and also to consider therapeutic modalities comprising of multiple distinct glycoforms.

In summary, the comprehensive CHO glycoengineering performed and the design matrix generated for lysosomal enzymes opens for systematic studies on options for improving ERTs by designed glycan features. Past studies have demonstrated the value of changing the structures of glycans on enzymes needed for ERTs, but the full potential has clearly not been met by use of yeast and plant production platforms or postproduction modification strategies. The CHO production platform offer new design capabilities, and the remarkable performance found for GLA capped with SA may represent a new design paradigm for many ERTs.

Methods

Establishment of stable CHO clones expressing recombinant human GLA and GBA enzymes

An expression construct containing the entire coding sequence of human GLA was synthesized by Genewiz, USA. Full-length cDNA of human GBA was purchased from Sino Biological Inc., China. Both constructs were subcloned into modified pCGS3 (Merck/Sigma-Aldrich) for glutamine selection in CHOZN GS−/− cells (Sigma). CHO cells were maintained as suspension cultures in serum-free media (EX-CELL CHO CD Fusion, cat. no. 14365C), supplemented with 4 mM l-glutamine in 50 mL TPP TubeSpin^® Bioreactors with 180 rpm shaking speed at 37 °C and 5% CO₂. Cells were seeded at 0.5 × 10⁶ cells/mL in T25 flask (NUNC, Denmark) one day prior to transfection. Electroporation was conducted with 2 × 10⁶ cells and 8 μg endotoxin-free plasmids using Amaxa Kit V and program U24 with Amaxa Nucleofector 2B (Lonza, Switzerland). Electroporated cells were subsequently plated in the 6-well plate with 3 mL growth media, and after 72 h, cells were plated in the 96-well plate at 1000 cells/well in 200 μL Minipool Plating Medium containing 80% EX-CELL^® CHO Cloning Medium (cat. no. C6366) and EX-CELL CHO CD Fusion serum-free media without glutamine. High expressing clones were selected by assaying the medium for enzyme activity (GLA) or with an enzyme-linked immunosorbent assay using anti-HIS antibodies (for GBA), and selected clones were scaled-up in serum-free media without l-glutamine in 50 mL TPP TubeSpin^® shaking Bioreactors (180 rpm, 37 °C and 5% CO₂) for enzyme production.

Purification of GLA and GBA

For GLA spent, the culture medium was centrifuged at 500 × g for 20 min, filtered (0.45 μm), diluted 3-fold with 25 mM MES (pH 6.0), and loaded onto a DEAE-Sepharose Fast Flow column (Sigma). The column was washed with 10 column volume (CV) washing buffer (25 mM MES with 50 mM NaCl, pH 6.0) and eluted with 5 CV elution buffer (25 mM MES with 200 mM NaCl, pH 6.0). For mouse studies. GLA was further purified by Mono-Q chromatography. For the HIS-tagged GBA culture, the medium was centrifuged, filtered, and mixed 3:1 (v/v) in 4× binding buffer (200 mM Tris, pH 8.0, 1.2 M NaCl), applied to 0.3 mL packed NiNTA agarose (Invitrogen), and then pre-equilibrated in the binding buffer (50 mM Tris, pH 8.0, 300 mM NaCl). The column was washed with the binding buffer and then eluted with 250 mM imidazole in the binding buffer. Purity and quantification was evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Coomassie staining.

CRISPR/Cas9-targeted KO in CHO cells

We designed and tested three to four guide RNAs (gRNAs) per gene with a high-throughput workflow⁶³. Green fluorescent protein (GFP)-tagged Cas9 nuclease was used to enrich for high Cas9 expression by fluorescence-activated cell sorting (FACS), and the cutting efficiency and indel profile of each gRNA was characterized by Indel Detection by Amplicon Analysis (IDAA)⁶⁴. We developed 43 validated gRNA constructs (Supplementary Table 1) and more than 200 CHO cell clones with different gene engineering design (Supplementary Table 2 and Supplementary Data 1). Gene editing was performed in CHO clones stably expressing GLA or GBA. Cells were seeded at 0.5 × 10⁶ cells/mL in T25 flask (NUNC, Denmark) one day prior to transfection, and 2 × 10⁶ cells and 1 μg each of endotoxin-free plasmid DNA of Cas9-GFP and gRNA in the plasmid U6GRNA (Addgene Plasmid #68370) were used for electroporation as described above. Forty eight hours after nucleofection, the 10–15% highest labeled (GFP) pool of cells were enriched by FACS, and after 1 week in culture, cells were single-cell sorted by FACS into the 96-well plate. KO clones were identified by IDAA as described⁶⁴, as well as when possible by immunocytology with appropriate lectins or monoclonal antibodies. Selected clones were further verified by Sanger sequencing. The strategy enabled fast screening and selection of KO clones with frameshift mutations, and on average we selected two to five clones from each targeting event. In general, the gene targeting did not substantially affect viability, growth, or productivity in the mutant cell clones. The full list of CRISPR gRNA design and PCR primers used are shown in Supplementary Table 1.

ZFN/CRISPR-mediated KI in CHO cells

We used targeted KI with zinc-finger nucleases (ZFNs) (modified ObLiGaRe⁶⁵ strategy) or CRISPR/Cas9-facilitated non-homologous end-joining⁶⁶ into a CHO Safe-Harbor locus^29,67. Site-specific CHO Safe-Harbor locus KI was based on ObLiGaRe strategy and performed with 2 µg of each ZFN (Merck/Sigma-Aldrich) tagged with GFP/Crimson²⁹, and 5 µg donor plasmid with full coding human genes (ST3GAL4, ST6GAL1, GNPTAB, or GNPTG). In brief, the EPB69 donor plasmid contained inverted CHO Safe-Harbor locus ZFN binding sites flanking the CMV promoter-ORF-BGH polyA terminator. Mono-allelic-targeted KI clones with one intact allele were selected by IDAA analysis⁶⁴. To stack a second gene into a Safe-Harbor locus, we first designed gRNA for the CHO Safe-Harbor locus flanking the ZFN binding site, followed by transfection with 1 µg of a donor PCR product of gene to be inserted with 1 μg Cas9-GFP and 1 μg gRNA. In brief, the donor PCR product was generated by using EPB69 donor plasmid as template, which contained the CMV promoter-ORF-BGH polyA terminator. KI clones were screened by PCR with primers specific for the junction area between the donor plasmid and the Safe-Harbor locus. A primer set flanking the targeted KI locus was used to characterize the allelic insertion status, and when possible, KI clones were also screened by immunocytology with lectins and monoclonal antibodies.

GLA enzyme activity assay

GLA enzyme activity was measured with 33 mM (unless otherwise specified) p-nitrophenyl-α-d-galactopyranoside at 37 °C for 30 min at pH 4.6 in 20 mM citrate and 30 mM sodium phosphate, and the reaction was quenched with borate buffer (pH 9.8) and released p-nitrophenol was read at 405 nm. A standard curve was generated by using 2-fold serial diluted p-nitrophenol in the same assay condition to calculate the amount of released product.

Site-specific N-glycopeptide analysis

Approximately 10 μg of purified GLA or GBA in 50 mM ammoniumbicarbonate buffer (pH 7.4) was reduced with dithiothreitol (10 mM) at 60 °C for 30 min and alkylated with iodoacetamide (20 mM) for 30 min in dark at room temperature. Chymotrypsin digestion was performed at a 1:25 enzyme–substrate ratio. The proteolytic digest was desalted by custom-made modified StageTip columns containing two layers of C18 and one layer of C8 membrane (3M Empore disks, Sigma-Aldrich)⁶⁸. Samples were eluted with 50% methanol in 0.1% formic acid, and then dried in SpeedVac and re-solubilized in 0.1% formic acid. Liquid chromatography MS/MS analysis was performed with an EASY-nLC 1000 LC system (Thermo Fisher Scientific) interfaced via nanoSpray Flex ion source to an Orbitrap Fusion MS (Thermo Fisher Scientific). Briefly, the nLC was operated in a single analytical column setup using PicoFrit Emitters (New Objectives, 75 μm inner diameter) custom packed with Reprosil-Pure-AQ C18 phase (Dr. Maisch, 1.9-μm particle size, 19–21 cm column length). Each sample was injected onto the column and eluted in a gradient from 2 to 25% of Solvent B for 45 min at 200 nL/min (Solvent A, 100% H₂O; Solvent B, 100% acetonitrile; both containing 0.1 % (v/v) formic acid). A precursor MS1 scan (m/z 350–2000) of intact peptides was acquired in the Orbitrap Fusion at the nominal resolution setting of 120,000, followed by Orbitrap HCD-MS2 at the nominal resolution setting of 60,000 of the five most abundant multiply charged precursors in the MS1 spectrum; a minimum MS1 signal threshold of 50,000 was used for triggering data-dependent fragmentation events. Targeted MS/MS analysis was performed by setting up a targeted MSⁿ (tMSⁿ) Scan Properties pane.

Glycopeptide compositional analysis was performed from m/z features using in-house written SysBioWare software⁶⁹. For m/z feature recognition from full MS scans LFQ Profiler Node of the Proteome discoverer 2.1 (Thermo Fisher Scientific) was used. A list of precursor ions (m/z, charge and retention time) was imported as ASCII data into SysBioWare and compositional assignment within 5 ppm mass tolerance was performed. The main building blocks used for the compositional analysis were: NeuAc, Hex, HexNAc, dHex, and phosphate. The most prominent peptides corresponding to each potential glycosite were added as an additional building block for compositional assignment. The most prominent peptide sequence related to each N-glycosite was determined experimentally by comparing the yield of deamidated peptides before and after PNGase F treatment. The peptide sequence was determined by higher-energy collisional dissociation MS/MS and the abundance level was calculated manually as the peak area from extracted ion chromatogram integrating ion current from at least four isotopes of the precursor ion envelope. A list of potential glycopeptides and glycoforms for each glycosite was generated and the top 10 of the most abundant candidates for each glycosite were selected for targeted MS/MS analysis to confirm the proposed structure. Each targeted MS/MS spectrum was subjected to manual interpretation. The same N-glycan composition may represent isobaric structures, so the listed glycan structure were assisted by and in agreement with the literature data predicting enzyme functions of the targeted genes together with useful information in MS/MS fragments.

Mouse studies

Fabry mice (~3.5 months male) and WT controls were used as reported previously¹⁷. All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee of Baylor Research Institute. All injections were performed via the tail vein with enzymes diluted in saline to a total volume of 200 μL per mouse, and the overall study design was identical to previous reports of studies with Fabrazyme and other GLA variants in this mouse model¹⁷.

Pharmacokinetics

Enzyme preparations were injected at a dose of 1 mg/kg body weight. At indicated time points, blood samples were collected from the tail vein, plasma was separated by centrifugation, and then used for enzyme assay¹⁷. SDS-PAGE Western blot for plasma GLA was conducted with rabbit polyclonal antibody to human GLA (Sigma, HPA000237, 1:1000 dilution) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulins (Dako, P0448, 1:3000 dilution).

Biodistribution and tissue kinetics

Enzyme preparations were injected at a dose of 1 mg/kg body weight. In a separate study, we used doses of 0.5 and 0.2 mg/kg as indicated. At indicated time points, mice were perfused with saline (to remove blood), and heart, kidney, liver, and spleen were dissected. The whole organs were homogenized in 0.2 % Triton/saline for enzyme assay. Protein concentration was measured using the BCA Protein Assay Kit (Pierce).

Immunohistochemistry

Enzyme preparations were injected at a dose of 2 mg/kg body weight. Heart, kidney, and liver were harvested 24 h after enzyme infusion. Untreated Fabry mouse tissues were used as negative controls. Tissues were fixed in formalin, embedded in paraffin, and 5-μm sections were made. IHC was performed by the Histopathology and Tissue Shared Resource in Georgetown University (Washington, DC, USA). In brief, after heat-induced epitope retrieval in citrate buffer, sections were treated with 3% hydrogen peroxide and 10% normal goat serum, and were then incubated with rabbit polyclonal antibody to human GLA (Sigma, cat. no. HPA000237) used at 1:300 dilution. After incubation with HRP-labeled polymer conjugated to goat anti-rabbit IgG (Dako, cat. no. K4003), signals were detected by DAB chromogen, and the sections were counterstained with hematoxylin. Signal specificity was verified with control staining, in which the primary antibody incubation was omitted. We also developed a mouse monoclonal antibody 6G8 to human GLA that was used to verify IHC. Briefly purified GLA was used for immunization of mice to develop hybridomas, and the specificity of 6G8 was evaluated in HEK293 cell with and without KO GLA.

Analysis of Gb3 in organs

Enzyme preparations or vehicle alone (saline) were injected into 6-month-old female Fabry mice at doses of 1 mg/kg body weight. Heart, kidney, and liver were harvested 2 weeks after a single injection. Tissue Gb3 levels were analyzed by MS as described¹⁷. In a separate study, we used doses of 0.5 and 0.2 mg/kg as indicated.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

All mass spectrometry raw data underlying Figs. 1–4 and Supplementary Figs. 2 and 4 have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD013140 [https://www.ebi.ac.uk/pride/archive/projects/PXD013140]. The source data underlying Fig. 5b–e, g and Supplementary Figs. 1, 3a–d, 6a–c, 7, 8 are provided as a Source Data file. Other data that support the findings of this study are available from the corresponding author upon request.

References

Neufeld, E. F. Lysosomal storage diseases. Annu. Rev. Biochem. 60, 257–280 (1991).
Article CAS Google Scholar
Futerman, A. H. & van Meer, G. The cell biology of lysosomal storage disorders. Nat. Rev. Mol. Cell. Biol. 5, 554–565 (2004).
Article CAS Google Scholar
Parenti, G., Pignata, C., Vajro, P. & Salerno, M. New strategies for the treatment of lysosomal storage diseases (review). Int. J. Mol. Med 31, 11–20 (2013).
Article CAS Google Scholar
Desnick, R. J. & Schuchman, E. H. Enzyme replacement therapy for lysosomal diseases: lessons from 20 years of experience and remaining challenges. Annu. Rev. Genom. Hum. Genet. 13, 307–335 (2012).
Article CAS Google Scholar
Grubb, J. H., Vogler, C. & Sly, W. S. New strategies for enzyme replacement therapy for lysosomal storage diseases. Rejuvenation Res. 13, 229–236 (2010).
Article CAS Google Scholar
Ghosh, P., Dahms, N. M. & Kornfeld, S. Mannose 6-phosphate receptors: new twists in the tale. Nat. Rev. Mol. Cell. Biol. 4, 202–212 (2003).
Article CAS Google Scholar
Stockert, R. J. The asialoglycoprotein receptor: relationships between structure, function, and expression. Physiol. Rev. 75, 591–609 (1995).
Article CAS Google Scholar
Martinez-Pomares, L. The mannose receptor. J. Leukoc. Biol. 92, 1177–1186 (2012).
Article CAS Google Scholar
Sly, W. S. et al. Enzyme therapy in mannose receptor-null mucopolysaccharidosis VII mice defines roles for the mannose 6-phosphate and mannose receptors. Proc. Natl Acad. Sci. USA 103, 15172–15177 (2006).
Article ADS CAS Google Scholar
Drickamer, K. & Taylor, M. E. Recent insights into structures and functions of C-type lectins in the immune system. Curr. Opin. Struct. Biol. 34, 26–34 (2015).
Article CAS Google Scholar
Macauley, M. S., Crocker, P. R. & Paulson, J. C. Siglec-mediated regulation of immune cell function in disease. Nat. Rev. Immunol. 14, 653–666 (2014).
Article CAS Google Scholar
Markmann, S. et al. Lrp1/LDL receptor play critical roles in mannose 6-phosphate-independent lysosomal enzyme targeting. Traffic 16, 743–759 (2015).
Article CAS Google Scholar
Sethuraman, N. & Stadheim, T. A. Challenges in therapeutic glycoprotein production. Curr. Opin. Biotechnol. 17, 341–346 (2006).
Article CAS Google Scholar
Brady, R. O., Murray, G. J. & Barton, N. W. Modifying exogenous glucocerebrosidase for effective replacement therapy in Gaucher disease. J. Inherit. Metab. Dis. 17, 510–519 (1994).
Article CAS Google Scholar
Tiels, P. et al. A bacterial glycosidase enables mannose-6-phosphate modification and improved cellular uptake of yeast-produced recombinant human lysosomal enzymes. Nat. Biotechnol. 30, 1225–1231 (2012).
Article CAS Google Scholar
Kizhner, T. et al. Characterization of a chemically modified plant cell culture expressed human alpha-galactosidase-A enzyme for treatment of Fabry disease. Mol. Genet. Metab. 114, 259–267 (2015).
Article CAS Google Scholar
Shen, J. S. et al. Mannose receptor-mediated delivery of moss-made alpha-galactosidase A efficiently corrects enzyme deficiency in Fabry mice. J. Inherit. Metab. Dis. 39, 293–303 (2016).
Article CAS Google Scholar
Barton, N. W. et al. Replacement therapy for inherited enzyme deficiency–macrophage-targeted glucocerebrosidase for Gaucher’s disease. N. Engl. J. Med. 324, 1464–1470 (1991).
Article CAS Google Scholar
Mistry, P. K. et al. Gaucher disease: progress and ongoing challenges. Mol. Genet. Metab. 120, 8–21 (2017).
Article CAS Google Scholar
Zimran, A. et al. Phase 1/2 and extension study of velaglucerase alfa replacement therapy in adults with type 1 Gaucher disease: 48-month experience. Blood 115, 4651–4656 (2010).
Article CAS Google Scholar
Zimran, A. et al. Pivotal trial with plant cell-expressed recombinant glucocerebrosidase, taliglucerase alfa, a novel enzyme replacement therapy for Gaucher disease. Blood 118, 5767–5773 (2011).
Article CAS Google Scholar
Zhu, Y. et al. Carbohydrate-remodelled acid alpha-glucosidase with higher affinity for the cation-independent mannose 6-phosphate receptor demonstrates improved delivery to muscles of Pompe mice. Biochem. J. 389, 619–628 (2005).
Article CAS Google Scholar
Basile, I. et al. Efficient therapy for refractory Pompe disease by mannose 6-phosphate analogue grafting on acid alpha-glucosidase. J. Control Rel. 269, 15–23 (2018).
Article CAS Google Scholar
Kang, J. Y. et al. Lysosomal targeting enhancement by conjugation of glycopeptides containing mannose-6-phosphate glycans derived from glyco-engineered Yeast. Sci. Rep. 8, 8730 (2018).
Article ADS Google Scholar
Desnick, R. J., Dean, K. J., Grabowski, G., Bishop, D. F. & Sweeley, C. C. Enzyme therapy in Fabry disease: differential in vivo plasma clearance and metabolic effectiveness of plasma and splenic alpha-galactosidase A isozymes. Proc. Natl Acad. Sci. USA 76, 5326–5330 (1979).
Article ADS CAS Google Scholar
Grubb, J. H. et al. Chemically modified beta-glucuronidase crosses blood-brain barrier and clears neuronal storage in murine mucopolysaccharidosis VII. Proc. Natl Acad. Sci. USA 105, 2616–2621 (2008).
Article ADS CAS Google Scholar
Rozaklis, T. et al. Impact of high-dose, chemically modified sulfamidase on pathology in a murine model of MPS IIIA. Exp. Neurol. 230, 123–130 (2011).
Article CAS Google Scholar
Meng, Y., Sohar, I., Wang, L., Sleat, D. E. & Lobel, P. Systemic administration of tripeptidyl peptidase I in a mouse model of late infantile neuronal ceroid lipofuscinosis: effect of glycan modification. PLoS ONE 7, e40509 (2012).
Article ADS CAS Google Scholar
Yang, Z. et al. Engineered CHO cells for production of diverse, homogeneous glycoproteins. Nat. Biotechnol. 33, 842–844 (2015).
Article CAS Google Scholar
Lee, K. et al. A biochemical and pharmacological comparison of enzyme replacement therapies for the glycolipid storage disorder Fabry disease. Glycobiology 13, 305–313 (2003).
Article Google Scholar
Huffaker, T. C. & Robbins, P. W. Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J. Biol. Chem. 257, 3203–3210 (1982).
CAS PubMed Google Scholar
Burda, P. & Aebi, M. The dolichol pathway of N-linked glycosylation. Biochim. Biophys. Acta 1426, 239–257 (1999).
Article CAS Google Scholar
Chavez, C. A. et al. Domain 5 of the cation-independent mannose 6-phosphate receptor preferentially binds phosphodiesters (mannose 6-phosphate N-acetylglucosamine ester). Biochemistry 46, 12604–12617 (2007).
Article CAS Google Scholar
Di Lorenzo, G. et al. Lysosomal proteome and secretome analysis identifies missorted enzymes and their nondegraded substrates in mucolipidosis III mouse cells. Mol. Cell Proteom. 17, 1612–1626 (2018).
Article Google Scholar
Voss, M. et al. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J. 33, 2890–2905 (2014).
Article CAS Google Scholar
Do, H. et al. Human mannose 6-phosphate-uncovering enzyme is synthesized as a proenzyme that is activated by the endoprotease furin. J. Biol. Chem. 277, 29737–29744 (2002).
Article CAS Google Scholar
Solomon, M. & Muro, S. Lysosomal enzyme replacement therapies: historical development, clinical outcomes, and future perspectives. Adv. Drug Deliv. Rev. 118, 109–134 (2017).
Article CAS Google Scholar
Brumshtein, B. et al. Characterization of gene-activated human acid-beta-glucosidase: crystal structure, glycan composition, and internalization into macrophages. Glycobiology 20, 24–32 (2010).
Article CAS Google Scholar
Fischer, S., Handrick, R. & Otte, K. The art of CHO cell engineering: A comprehensive retrospect and future perspectives. Biotechnol. Adv. 33, 1878–1896 (2015).
Article CAS Google Scholar
Shaaltiel, Y. et al. Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement therapy of Gaucher’s disease using a plant cell system. Plant Biotechnol. J. 5, 579–590 (2007).
Article CAS Google Scholar
Bohnsack, R. N. et al. Cation-independent mannose 6-phosphate receptor: a composite of distinct phosphomannosyl binding sites. J. Biol. Chem. 284, 35215–35226 (2009).
Article CAS Google Scholar
Sakuraba, H. et al. Comparison of the effects of agalsidase alfa and agalsidase beta on cultured human Fabry fibroblasts and Fabry mice. J. Hum. Genet. 51, 180–188 (2006).
Article CAS Google Scholar
Park, E. I., Mi, Y., Unverzagt, C., Gabius, H. J. & Baenziger, J. U. The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid alpha 2,6GalNAc. Proc. Natl Acad. Sci. USA 102, 17125–17129 (2005).
Article ADS CAS Google Scholar
Unverzagt, C. et al. Structure-activity profiles of complex biantennary glycans with core fucosylation and with/without additional α2, 3/α2, 6 aialylation: synthesis of neoglycoproteins and their properties in lectin assays, cell binding, and organ uptake. J. Med. Chem. 45, 478–491 (2002).
Article CAS Google Scholar
Furbish, F. S., Steer, C. J., Barranger, J. A., Jones, E. A. & Brady, R. O. The uptake of native and desialylated glucocerebrosidase by rat hepatocytes and Kupffer cells. Biochem. Biophys. Res. Commun. 81, 1047–1053 (1978).
Article CAS Google Scholar
Murray, G. J., Anver, M. R., Kennedy, M. A., Quirk, J. M. & Schiffmann, R. Cellular and tissue distribution of intravenously administered agalsidase alfa. Mol. Genet. Metab. 90, 307–312 (2007).
Article CAS Google Scholar
Ioannou, Y. A., Zeidner, K. M., Gordon, R. E. & Desnick, R. J. Fabry disease: preclinical studies demonstrate the effectiveness of alpha-galactosidase A replacement in enzyme-deficient mice. Am. J. Hum. Genet. 68, 14–25 (2001).
Article CAS Google Scholar
Damme, M. et al. Chronic enzyme replacement therapy ameliorates neuropathology in alpha-mannosidosis mice. Ann. Clin. Transl. Neurol. 2, 987–1001 (2015).
Article CAS Google Scholar
Platt, F. M. Emptying the stores: lysosomal diseases and therapeutic strategies. Nat. Rev. Drug Discov. 17, 133–150 (2018).
Article CAS Google Scholar
Parenti, G., Andria, G. & Ballabio, A. Lysosomal storage diseases: from pathophysiology to therapy. Annu. Rev. Med. 66, 471–486 (2015).
Article CAS Google Scholar
Dahms, N. M., Lobel, P. & Kornfeld, S. Mannose 6-phosphate receptors and lysosomal enzyme targeting. J. Biol. Chem. 264, 12115–12118 (1989).
CAS PubMed Google Scholar
Furbish, F. S., Steer, C. J., Krett, N. L. & Barranger, J. A. Uptake and distribution of placental glucocerebrosidase in rat hepatic cells and effects of sequential deglycosylation. Biochim. Biophys. Acta 673, 425–434 (1981).
Article CAS Google Scholar
Grabowski, G. A. et al. Enzyme therapy in type 1 Gaucher disease: comparative efficacy of mannose-terminated glucocerebrosidase from natural and recombinant sources. Ann. Intern. Med. 122, 33–39 (1995).
Article CAS Google Scholar
Liu, L., Lee, W. S., Doray, B. & Kornfeld, S. Engineering of GlcNAc-1-phosphotransferase for production of highly phosphorylated lysosomal enzymes for enzyme replacement therapy. Mol. Ther. Methods Clin. Dev. 5, 59–65 (2017).
Article CAS Google Scholar
Wang, D. et al. Engineering a lysosomal enzyme with a derivative of receptor-binding domain of apoE enables delivery across the blood–brain barrier. Proc. Natl Acad. Sci. USA 110, 2999–3004 (2013).
Article ADS CAS Google Scholar
Maga, J. A. et al. Glycosylation-independent lysosomal targeting of acid alpha-glucosidase enhances muscle glycogen clearance in pompe mice. J. Biol. Chem. 288, 1428–1438 (2013).
Article CAS Google Scholar
Stroobants, S. et al. Long-term enzyme replacement therapy improves neurocognitive functioning and hippocampal synaptic plasticity in immune-tolerant alpha-mannosidosis mice. Neurobiol. Dis. 106, 255–268 (2017).
Article CAS Google Scholar
Markmann, S. et al. Quantitative proteome analysis of mouse liver lysosomes provides evidence for mannose 6-phosphate-independent targeting mechanisms of acid hydrolases in mucolipidosis II. Mol. Cell. Proteom. 16, 438–450 (2017).
Article CAS Google Scholar
Prabakaran, T. et al. Mannose 6-phosphate receptor and sortilin mediated endocytosis of alpha-galactosidase A in kidney endothelial cells. PLoS ONE 7, e39975 (2012).
Article ADS CAS Google Scholar
Prabakaran, T. et al. Receptor-mediated endocytosis of alpha-galactosidase A in human podocytes in Fabry disease. PLoS ONE 6, e25065 (2011).
Article ADS CAS Google Scholar
Xu, S. et al. Coformulation of a novel human alpha-galactosidase A with the pharmacological chaperone AT1001 leads to improved substrate reduction in fabry mice. Mol. Ther. 23, 1169–1181 (2015).
Article CAS Google Scholar
Benjamin, E. R. et al. Co-administration with the pharmacological chaperone AT1001 increases recombinant human alpha-galactosidase A tissue uptake and improves substrate reduction in Fabry mice. Mol. Ther. 20, 717–726 (2012).
Article CAS Google Scholar
Narimatsu, Y. et al. A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Glycobiology 28, 295–305 (2018).
Article CAS Google Scholar
Yang, Z. et al. Fast and sensitive detection of indels induced by precise gene targeting. Nucleic Acids Res. 43, e59 (2015).
Article Google Scholar
Maresca, M., Lin, V. G., Guo, N. & Yang, Y. Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining. Genome Res. 23, 539–546 (2013).
Article CAS Google Scholar
Geisinger, J. M., Turan, S., Hernandez, S., Spector, L. P. & Calos, M. P. In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Nucleic Acids Res. 44, e76 (2016).
Article Google Scholar
Bahr, S., Cortner, L., Ladley, S. & Borgschulte, T. Evaluating the effect of chromosomal context on zinc finger nuclease efficiency. BMC Proc. 7 (Suppl. 6), P3 (2013).
Rappsilber, J., Mann, M. & Ishihama, Y. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat. Protoc. 2, 1896–1906 (2007).
Article CAS Google Scholar
Vakhrushev, S. Y., Dadimov, D. & Peter-Katalinic, J. Software platform for high-throughput glycomics. Anal. Chem. 81, 3252–3260 (2009).
Article CAS Google Scholar
Varki, A. et al. Symbol nomenclature for graphical representations of glycans. Glycobiology 25, 1323–1324 (2015).
Article CAS Google Scholar

Download references

Acknowledgements

We thank Thomas Braulke (Hamburg) for discussions and critical reading of the manuscript. This work was supported by the Lundbeck Foundation, Novo Nordisk Foundation, Kirsten og Freddy Johansen Fonden, A.P. Møller Fonden, Læge Sofus Carl Emil Friis og hustru Olga Doris Friis’ Legat, Innovation Fund Denmark, and the Danish National Research Foundation (DNRF107).

Author information

Authors and Affiliations

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 N, Copenhagen, Denmark
Weihua Tian, Zilu Ye, Shengjun Wang, Morten Alder Schulz, Julie Van Coillie, Lingbo Sun, Yen-Hsi Chen, Yoshiki Narimatsu, Lars Hansen, Ulla Mandel, Eric Paul Bennett, Sergey Y. Vakhrushev, Henrik Clausen & Zhang Yang
GlycoDisplay ApS, Blegdamsvej 3, 2200, Copenhagen N, Denmark
Claus Kristensen
Institute of Metabolic Disease, Baylor Scott & White Research Institute, 3812 Elm Street, Dallas, TX, 75226, USA
Siamak Jabbarzadeh-Tabrizi, Raphael Schiffmann & Jin-Song Shen

Authors

Weihua Tian
View author publications
You can also search for this author in PubMed Google Scholar
Zilu Ye
View author publications
You can also search for this author in PubMed Google Scholar
Shengjun Wang
View author publications
You can also search for this author in PubMed Google Scholar
Morten Alder Schulz
View author publications
You can also search for this author in PubMed Google Scholar
Julie Van Coillie
View author publications
You can also search for this author in PubMed Google Scholar
Lingbo Sun
View author publications
You can also search for this author in PubMed Google Scholar
Yen-Hsi Chen
View author publications
You can also search for this author in PubMed Google Scholar
Yoshiki Narimatsu
View author publications
You can also search for this author in PubMed Google Scholar
Lars Hansen
View author publications
You can also search for this author in PubMed Google Scholar
Claus Kristensen
View author publications
You can also search for this author in PubMed Google Scholar
Ulla Mandel
View author publications
You can also search for this author in PubMed Google Scholar
Eric Paul Bennett
View author publications
You can also search for this author in PubMed Google Scholar
Siamak Jabbarzadeh-Tabrizi
View author publications
You can also search for this author in PubMed Google Scholar
Raphael Schiffmann
View author publications
You can also search for this author in PubMed Google Scholar
Jin-Song Shen
View author publications
You can also search for this author in PubMed Google Scholar
Sergey Y. Vakhrushev
View author publications
You can also search for this author in PubMed Google Scholar
Henrik Clausen
View author publications
You can also search for this author in PubMed Google Scholar
Zhang Yang
View author publications
You can also search for this author in PubMed Google Scholar

Contributions

W.T. designed, planned, and performed most of the gene targeting experiments and co-wrote the manuscript. Zi.Y. and S.Y.V. performed the site-specific glycan analyses. S.W., M.A.S., J.V.C., L.S., Y-H.C. and Y.N. contributed to gene targeting experiments. L.H., C.K. and E.P.B. contributed to the design and analyses of experiments. U.M. contributed to antibody development. S.J-T., R.S. and J.-S.S. performed the mouse studies. H.C. and Z.Y. designed, planned, and co-wrote the manuscript.

Corresponding authors

Correspondence to Henrik Clausen or Zhang Yang.

Ethics declarations

Competing interests

The University of Copenhagen has filed patent applications EP/3455635 and US/62724543 partly on the basis of this work. W.T., Y.N., C.K., U.M., E.P.B., S.Y.V., Z.Y. and H.C. are named inventors on one or both of these applications. GlycoDisplay ApS has license rights to the patent applications. W.T., Z.Y., Y.N., E.P.B., C.K. and H.C. have shares in GlycoDisplay Aps. C.K. is an employee of GlycoDisplay ApS. The remaining authors declare no competing interests.

Additional information

Journal peer review information: Nature Communications thanks Leander Meuris and other anonymous reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available.

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Supplementary Information

Peer Review File

Description of Additional Supplementary Files

Supplementary Data 1

Reporting Summary

Source Data

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Cite this article

Tian, W., Ye, Z., Wang, S. et al. The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells. Nat Commun 10, 1785 (2019). https://doi.org/10.1038/s41467-019-09809-3

Download citation

Received: 17 August 2018
Accepted: 29 March 2019
Published: 30 April 2019
DOI: https://doi.org/10.1038/s41467-019-09809-3

This article is cited by

Optimizing human α-galactosidase for treatment of Fabry disease
- William C. Hallows
- Kristen Skvorak
- Gjalt W. Huisman
Scientific Reports (2023)
Development of a Biosimilar of Agalsidase Beta for the Treatment of Fabry Disease: Preclinical Evaluation
- André B. P. van Kuilenburg
- Carla E. M. Hollak
- Mariana Papouchado
Drugs in R&D (2023)
Identification of global inhibitors of cellular glycosylation
- Daniel Madriz Sørensen
- Christian Büll
- Yoshiki Narimatsu
Nature Communications (2023)
N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion
- Laura Hobohm
- Tomas Koudelka
- Matthias Voss
Cellular and Molecular Life Sciences (2022)
Organelle-level precision with next-generation targeting technologies
- Anand Saminathan
- Matthew Zajac
- Yamuna Krishnan
Nature Reviews Materials (2021)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.