Fig. 6 | Nature Communications

Fig. 6

From: The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration

Fig. 6

Fam3a rescues impaired commitment of Stat3 knockout (KO) muscle stem cells (MuSCs) in vitro and in vivo. a Immunofluorescence analysis and quantification of myogenin expression in MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 3 independent experiments). Scale bar 25 μm. b Immunofluorescence analysis and quantification of myogenin expression in control and Stat3 KO MuSCs cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). Scale bar 25 μm. ce Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of Stat3 KO MuSCs cultured in growth conditions for 3 days in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). The Cell Mito Stress Test was performed using the Seahorse XFp Analyzer. f Scheme of the experimental design for the intramuscular Fam3a treatment in vivo. g, h Immunofluorescence analysis and quantification of Pax7- and myogenin-positive cells in transversal sections of the tibialis anterior muscles of Pax7-CreERWT;Stat3f/f and Pax7-CreER;Stat3f/f male mice 6 days after barium chloride injury. Vehicle or 1 μg of Fam3a recombinant protein was injected intramuscularly 1 day after inducing injury (n = 3–4 animals). Scale bar 25 μm. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, **p < 0.01, ****p < 0.0001)

Back to article page