Fig. 3 | Nature Communications

Fig. 3

From: The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration

Fig. 3

Fam3a promotes muscle stem cell (MuSC) myogenic lineage progression in vitro. a Gene expression analysis of MuSCs infected with lentiviruses coding for short hairpin RNA (shRNA) Control (shCt) or shRNA against Fam3a (shFam3a) and cultured in growth conditions for 72 h (n = 4 independent experiments). b, c Immunofluorescence analysis and quantification of myogenic markers of MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in growth conditions for 72 h (n = 3 independent experiments). Scale bars 50 μm. d Immunofluorescence analysis of myogenic differentiation of MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in differentiation conditions for 72 h (n = 3 independent experiments). Scale bar 100 μm. Data are represented as mean ± SEM (Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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