Fig. 1 | Nature Communications

Fig. 1

From: The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration

Fig. 1

Stat3 promotes mitochondrial function in muscle stem cells (MuSCs) during activation. a Scheme of the experimental design to obtain the samples for RNA-seq. b Heat map of the differentially expressed genes among the different comparisons. Listed genes were differentially expressed in at least one of the comparisons. Normalized gene RPKM (reads per kilobase of transcript per million) values were averaged within groups for the generation of the heat map (n = 3 animals). c Pie charts showing the top 20 differentially enriched pathways (using Gene Set Enrichment Analysis (GSEA)) during the activation of control (Pax7-CreERWT;Stat3f/f) or Stat3 KO MuSCs (Pax7-CreER;Stat3f/f mice) grouped according to their functional category. d Top 12 pathways enriched in freshly isolated MuSCs from 3 dpi Pax7-CreER;Stat3f/f mice compared to 3 dpi MuSCs isolated from control littermates using GSEA. The red line indicates p value = 0.05 based on the pathway analysis (GSEA). eg Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of control and Stat3 KO MuSCs cultured in growth conditions for 3 days (n = 4 independent experiments). The Cell Mito Stress Test was performed in a Seahorse XFp Analyzer. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, ***p < 0.001, ****p < 0.0001)

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