Fig. 5 | Nature Communications

Fig. 5

From: Gut microbiome-derived phenyl sulfate contributes to albuminuria in diabetic kidney disease

Fig. 5

Effect of 2-aza-tyrosine (AZA) on the gut microbiome in adenine-induced renal failure mice. a Observed OTUs rarefaction analysis. OTU rarefaction curves of gut microbiome were used to estimate richness in the following groups: control mice (Ctrl); control mice treated with 5 mg/kg 2-aza-tyrosine (AZA); adenine-induced renal failure mice (RF); and adenine-induced renal failure mice treated with 5 mg/kg 2-aza-tyrosine (RF-AZA). b OTU-based α-diversity of each microbiome. No significant diversity was seen in any group by Chao1 (left) and Shannon (right) analyses. c Principal coordinates analysis of the microbiome profiles using weighted UniFrac. Scores are presented for the first principal component (PCo1) vs. both the second principal component (PCo2) and the third principal component (PCo3). d Relative abundance of microbiota based on the average number of each subgroup at order, family and genus levels. The major subgroups are indicated on the right. e The relative abundance of microbiota differed significantly among the four groups analyzed. The y-axis indicates the relative abundance of each microbe as a percentage. The proportional change in the minor groups correlated with renal failure and 2-aza-tyrosine treatment. The major microbial order (Bacteroidales, Clostridales, and Lactobacillales) were unchanged by either renal failure or 2-aza-tyrosine treatment. However, the minor populations (Coriobacteriales and Erysipelotrichales) were significantly changed in the correlation with the PS concentration by renal failure and 2-aza-tyrosine treatment. n = 5 in each group. Data were mean ± SEM. Statistical analysis was performed by the Tukey–Kramer test, followed by FDR correction of p values. *pFDR < 0.1 was treated as statistically significant. The following groups were tested: control group (Ctrl); control group administered 2-aza-tyrosine (AZA); the renal failure group (RF); and the renal failure group administered 2-aza-tyrosine (RF-AZA). Metagenome Source data are provided as in a Source Data file

Back to article page