Fig. 6 | Nature Communications

Fig. 6

From: Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance

Fig. 6

TFEB S142 phosphorylation promotes the dissemination of BRAFV600E melanoma cells. a Representative gross images (top row), H&E-stained section (second row), and immunohistochemical (IHC) staining of p62 and p-Smad3 in lung metastasis of B16-F10 melanoma cells stably expressing BRAFV600E along with the indicated TFEB proteins. T metastatic melanoma tumors. b Quantification of the numbers of lung metastasis formed by B16-F10 melanoma cells as indicated (n= 5–6 mice per group; data represent mean ± SD). Comparisons were made (vs. Vector group) using Student’s t-test. ***P < 0.001. c Immunoblot analyses of E-cadherin, N-cadherin, Vimentin, active TGF-β, LC3 conversion, and p62 in indicated lung metastases (two randomly chosen samples per group; similar results were observed in all 10–12 samples per condition). Actin served as a loading control. n= 3 independent experiments. See Supplementary Fig. 13 for uncropped data. Scale bars, 500 μm. Data in c are from one experiment that is representative of three independent experiments. For all quantification, data represent the mean ± SD derived from the indicated number of independent experiments. Comparisons were made using Student’s t-test. ***P < 0.001