Table 4 Summary of IL2RGsgRNA off-target INDEL frequency analysis

From: Gene correction for SCID-X1 in long-term hematopoietic stem cells

Gene name Guide Seq COSMID Chromosome location Features Expression in HSCsa U2OS (plasmid) WT CD34+ 20 nt RNP (WT Cas9) SCID-X1 CD34+ 19 nt RNP (WT Cas9) SCID-X1 CD34+ 20 nt RNP (HiFi Cas9) SCID-X1 CD34+19 nt RNP (HiFi Cas9)
IL2RG    X Exon Yes 81.1% 81.7% 91.7% 94.1% 97.6%
LIN01287   7 Intergenic Data not available Background Background Background Not sequenced Not sequenced
MPZL1   1 Intron Yes 1.1% 0.1% 0.1% Background Background
SHQ1 3 Intron Yes 1.5% Background Background Not sequenced Not sequenced
SMYD3   1 Intron Yes 4.2% Background Background Not sequenced Not Sequenced
ZNF330   4 Intergenic Data not available Background 0.23% 0.27% Background Background
  1. aExpression determined by www.biogps.org and Gene Expression Commons