Fig. 4 | Nature Communications

Fig. 4

From: Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK–NF-κB signaling mediated inflammation

Fig. 4

MVP deficiency promotes inflammation in HFD-fed mice. a Representative CD68+ staining in epiWAT from HFD-fed WT and MVP KO mice (n = 5). Scale bars, 50 μm. b, c Quantification of epiWAT SVFs (b) and macrophages (c) by flow cytometry in HFD-fed WT and MVP KO mice (n = 6). d Representative flow cytometry plot charts of F4/80+CD11b+ macrophages in epiWAT of HFD-fed WT and MVP KO mice. ei mRNA levels of inflammatory mediators in epiWAT (e), subWAT (f), BAT (g), liver (h) (n = 6–8), and epiWAT F4/80+ macrophages (n = 3) (i) in HFD-fed WT and MVP KO mice. j Plasma concentrations of TNF-α, IL-6, IL-1β, and CCL2 in HFD-fed WT and MVP KO mice (n = 8). k Representative CD68+ staining in epiWAT from HFD-fed MacWT and MacKO mice (n = 5). Scale bars, 50 μm. l, m Quantification of epiWAT SVFs (l) and macrophages (m) by flow cytometry in HFD-fed MacWT and MacKO mice (n = 6). n Representative flow cytometry plot charts of F4/80+CD11b+ macrophages in epiWAT of HFD-fed MacWT and MacKO mice. os mRNA levels of inflammatory mediators in epiWAT (o), subWAT (p), BAT (q), liver (r) (n = 6–8), and epiWAT F4/80+ macrophages (n = 3) (s) in HFD-fed MacWT and MacKO mice. t Plasma concentrations of TNF-α, IL-6, IL-1β, and CCL2 in HFD-fed MacWT and MacKO mice (n = 8). Data are expressed as mean ± SEM. *P < 0.05 and **P < 0.01 by Student’s t test

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