Fig. 1 | Nature Communications

Fig. 1

From: Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK–NF-κB signaling mediated inflammation

Fig. 1

MVP expression is up-regulated in macrophages from obese mice and human beings. Male C57BL/6J mice were fed a CD or a HFD for 12 weeks. a Western blot analysis of MVP expression in epiWAT (n = 6). b IHC staining of MVP in epiWAT. Scale bars, 50 μm (top) and 20 μm (bottom). c Western blot analysis of MVP expression in the adipocytes and SVFs isolated from epiWAT (n = 3). d Immunofluorescence images of staining with antibodies against CD68 (green), MVP (red), and Perilipin (purple) in epiWAT of HFD-fed mice. Nuclei were stained with DAPI (blue). Scale bars, 20 μm. e Western blot analysis of MVP expression in sorted F4/80+ macrophages isolated from epiWAT SVFs (n = 3). f Western blot analysis of MVP in PMs (n = 6). g, h IHC staining (g) and quantitative analysis (h) of MVP in the visceral adipose tissue from normal weight donors and individuals with overweight or obesity (n = 5). Scale bars, 50 μm (top) and 20 μm (bottom). i Representative immunofluorescence images of staining with antibodies against CD68 (green), MVP (red), and Perilipin (purple) in visceral adipose tissue of overweight or obese individuals. Nuclei were stained with DAPI (blue). Scale bars, 20 μm. j mRNA level of MVP in CD14+ macrophages isolated from the visceral adipose tissue SVFs of normal weight (18.5 ≤ BMI < 24, n = 15) and overweight (24 ≤ BMI < 28) or obese (BMI ≥ 28) (n = 24) subjects. Data are expressed as mean ± SEM. *P < 0.05 and **P < 0.01 by Student’s t test or ANOVA with post hoc test

Back to article page