Table 2 Binding Data of CaM Species for NaV1.4 or NaV1.5 CTerm

From: Ca2+-dependent regulation of sodium channels NaV1.4 and NaV1.5 is controlled by the post-IQ motif

Sample Cell [Ca2+] mM Titrant Kd nM −ΔG Kcal mol−1
Nav1.4 CTerm Long 0 apoCaM 17 ± 3 10.59
Nav1.4 CTerm Long 1 (Ca2+)4–CaM 275 ± 26 8.94
Nav1.4 CTerm Long 1 (Ca2+)2-C–CaM CaM12 154 ± 9 9.29
Nav1.4 CTerm Long 1 (Ca2+)2-N–CaM CaM34 121 ± 7 9.43
Nav1.5 CTerm Long 0 apoCaM 48 ± 5 9.98
Nav1.5 CTerm Long 1 (Ca2+)4–CaM 89 ± 7 9.61
Nav1.5 CTerm Long 1 (Ca2+)2-C–CaM CaM12 3600 ± 200 7.43
Nav1.5 CTerm Long 1 (Ca2+)2-N–CaM CaM34 90 ± 4 9.58
Nav1.4 CTerm Short 0 apoCaM 114 ± 11 9.47
Nav1.4 CTerm Short 1 (Ca2+)4–CaM 305 ± 21 8.88
Nav1.4 CTerm Short 1 (Ca2+)2-C–CaM CaM12 156 ± 5 9.28
Nav1.4 CTerm Short 1 (Ca2+)2-N–CaM CaM34 282 ± 6 8.93
Nav1.5 CTerm Short 0 apoCaM 39 ± 3 10.10
Nav1.5 CTerm Short 1 (Ca2+)4–CaM 1520 ± 90 7.93
Nav1.5 CTerm Short 1 (Ca2+)2-C–CaM CaM12 5260 ± 240 7.20
Nav1.5 CTerm Short 1 (Ca2+)2-N–CaM CaM34 376 ± 12 8.76