Fig. 8 | Nature Communications

Fig. 8

From: In vivo topology converts competition for cell-matrix adhesion into directional migration

Fig. 8

Sdf1 does not need to interact with Fibronectin to rescue the effect of Sema3A. a Xenopus NC cells cultured on Fibronectin or Matrigel with or without Sema3A coated at 60 ng/mL and/or Sdf1 added in solution at 0.5 μg/mL, stained with DAPI and Phalloidin. bd Normalised cell area (b), aspect ratio (c) and circularity (d) per cell for each condition shown in (a). For b, c and d, n = 160 cells (FN: n = 30, Matrigel, ncontrols = 38, nSdf = 69, nSema = 7 and nSdf+Sema = 16). Since only seven cells remained attached in the Sema3A condition we did not perform statistical analysis. e Mouse neural crest cell line, 09–1, cultured on Matrigel with or without Sema3A coated at 60 ng/mL and/or Sdf1 at 0.5 μg/mL and/or Mn2+ (2 mM) added in solution, stained with DAPI and Phalloidin. fh Normalised cell area (f), aspect ratio (g) and circularity (h) per cell for each condition shown in e. n = 1334 cells (ncontrols = 370, nSema = 268, nSdf = 96, nSema+Mn = 224 and nSema+Sdf = 376) from four independent experiments. ANOVA followed by multiple comparisons, ****p < 0.0001; ***p < 0.001; **p < 0.01. ik Normalised cell area (i), aspect ratio (j) and circularity (k) per cell for mouse NC cells cultured on Fibronectin with or without Sema3A coated at 60 ng/mL and/or Sdf1 (0.5 μg/mL and/or Mn2+ (2 mM) added in solution corresponding to experimental conditions shown in Supplementary Fig. 8. n = 3421 cells (ncontrols = 653, nSema = 804, nSdf = 675, nSema+Mn = 591 and nSema+Sdf = 698). ANOVA followed by multiple comparisons, ****p < 0.0001; ***p < 0.001. Scale bars in a, 10 μ; in e low magnification, 50 μ; in e high magnification, 10 μ. Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. Source data are provided as a Source Data file

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