Fig. 5 | Nature Communications

Fig. 5

From: In vivo topology converts competition for cell-matrix adhesion into directional migration

Fig. 5

Sdf1 counteracts Sema3A’s effects on single cell motility. a Tracks of single cells on Fibronectin or Fibronectin plus Sema3A. b, c Speed and Directionality in cells shown in a, n = 188 cells (ncontrols = 64; nSema3A = 53; nSema3F = 71). ANOVA, followed by multiple comparisons. d Tracks of single cells cultured on Fibronectin or Fibronectin plus Sema3A before and after Sdf1 was added in solution. e Speed of cells in conditions shown in d, ANOVA, followed by multiple comparisons; ns, p = 0.621; *p = 0.0188; ****p < 0.0001. f Directionality in cells shown in d, ANOVA, followed by multiple comparisons; *p = 0.0331. Note that Sdf1 reduces slightly speed and increases directionality whereas Sema3A strongly reduces both. Sdf1 restores control values when added to the Sema3A condition. n = 190 cells (ncontrols = 60; nSdf1 = 29; nSema3A = 73; nSema3A+Sdf1 = 28). Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. Source data are provided as a Source Data file

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