Fig. 4 | Nature Communications

Fig. 4

From: Muscle functions as a connective tissue and source of extracellular matrix in planarians

Fig. 4

hemicentin-1 (hmcn-1) is required for maintaining parenchymal neoblast localization. a hmcn-1 expression is strongest in the colF-2+ sub-epidermal body wall muscle. Double-positive cell number of total hmcn-1+ cells from 1 representative of four animals. Ph, pharynx. Lower panel: scale bar, 100 μm. b Inhibition of hmcn-1 leads to severe epidermal ruffling in live animals. Scale bar, 200 μm. Four experiments. c TEM micrograph of ventral supra-muscle region representative of four sections from two animals. Red line indicates distance from basal epidermal surface (Ep) to circular muscle fiber (CF) layer. White line indicates area of high density ECM characterizing the sub-epidermal membrane (SM). Dorsal, up. Ep, epidermis. LF, longitudinal muscle fiber. d Dividing (H3P+) neoblasts (smedwi-1+) and cells of epidermal lineage (prog-2+, agat-3+) are found ectopically between the 6G10+ muscle fibers and the ventral epidermis. Left panel: total smedwi-1+ cells (magenta) outside or inside muscle fibers in the post-pharyngeal region counted in four animals. e Confocal imaging of different ectopic cells underneath the epidermis. Bottom panel shows cryosection. Scale bars, 100 μm. f Neoblast (NB) subpopulations were present outside of the 6G10+ muscle layer in hmcn-1(RNAi) animals. Right panels show white-boxed areas with and without smedwi-1 channel. Left panels, scale bar 100 μm. White arrowhead, ectopic cells. *indicates interior of an intestinal branch. Unless specified, 36 days RNAi, fractions indicate number of total animals from two independent RNAi experiments with phenotype shown in representative image. Anterior is up, RNA probes for FISH of indicated gene, scale bars, 10 μm