Fig. 2 | Nature Communications

Fig. 2

From: Muscle functions as a connective tissue and source of extracellular matrix in planarians

Fig. 2

Collagen expression is specific to muscle cells. a tSNE dimensional reduction of gene expression in 50,562 cells12 showing major cell types in planarians. b Circles represent a protein-coding gene within the matrisome, colored by most enriched cell type (greatest AUC) and sized by confidence in ECM localization. c FISH using RNA probes of indicated collagen-encoding gene (magenta) with percentage of cells double positive for the canonical planarian muscle marker colF-2 (green). Red box in cartoon shows area imaged. Scale bars, 10 μm. d tSNE plot as in Fig. 2a with each cell colored and sized by the average normalized expression of high confidence core glycoprotein-encoding genes and collagen-encoding genes