The cDNA sequence of human SMART described in this Article was misreported, as described in the accompanying Addendum. This error does not affect the results or any conclusion of the Article.
Addendum to: Nature Communications https://doi.org/10.1038/s41467-018-06985-6, published online 26 October 2018
It has come to our attention that the human SMART biosensor reported in this Article does not contain SAGG repeats replacing the a and b regions corresponding to MLKL, as described. Following requests for murine and human SMART cDNAs, we were alerted that the sequence of human SMART is identical to human alpha15 (α15), which was a prototype of human SMART. We checked the sequence of the FRET biosensor integrated into HT29- and HaCaT cells, described in Figures 6 and 7, and found that it is human α15 and not our reported human SMART sequence (Figure. 1). To confirm that the human α15 construct behaves in the same way as the sensor described as human SMART, we regenerated stably transfected HT29 and HaCaT cells expressing the human α15 construct. As shown in Figure. 2, TNF+BV6+zVAD and PolyIC+BV6+zVAD induce an increase in the FRET/CFP ratio of human α15 in HT29 and HaCaT cells, respectively. This error does not affect the results or any conclusions of the Article.
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Murai, S., Yamaguchi, Y., Shirasaki, Y. et al. Addendum: A FRET biosensor for necroptosis uncovers two different modes of the release of DAMPs. Nat Commun 10, 1923 (2019). https://doi.org/10.1038/s41467-019-09536-9
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DOI: https://doi.org/10.1038/s41467-019-09536-9
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