Fig. 2 | Nature Communications

Fig. 2

From: Excessive mechanical loading promotes osteoarthritis through the gremlin-1–NF-κB pathway

Fig. 2

Catabolic effects of gremlin-1 in cultured chondrocytes. a mRNA levels of marker genes in primary mouse articular chondrocytes treated with recombinant human gremlin-1 (rhGREM1) for 24 h. b Amount of glycosaminoglycans (GAG) released into the culture medium determined by dimethylmethylene blue assay of wild-type mouse femoral heads cultured with various amounts of rhGREM1 for 3 days. c mRNA levels of marker genes in mouse femoral heads cultured with 10 µg/mL rhGREM1 for 3 days. All data are expressed as mean ± SD of biologically independent three samples per group. *P < 0.05, **P < 0.01, #P < 0.005, ##P < 0.001 versus 0 (vehicle) (one-way ANOVA test)

Back to article page