Fig. 3 | Nature Communications

Fig. 3

From: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif

Fig. 3

Inhibition of maize PLCPs by U. maydis and U. hordei Pit2/PID14. The activity of PLCPs in apoplastic fluids of maize leaves treated with salicylic acid (SA) was determined using the fluorogenic substrate Z-Phe-Arg-AMC. a Concentration range of inhibition using heterologous expressed UmPit2 and UhPit2 proteins. Activity was set to 100% in the absence of inhibitor. Curves were generated using a nonlinear fit and error bars are SEM of three independent replicates. b Activity of apoplastic PLCPs during infection. Apoplastic fluids of maize seedlings infected with U. maydis SG200, ΔPit2 and the complemented ΔPit2-UhPit2 and ΔPit2-UmPit2 were collected 3 dpi and protein quantification was performed. Apoplastic fluids were set to a concentration of 57 µg/ml total protein and PLCP activity was measured using the fluorogenic substrate Z-Phe-Arg-AMC. Values are means of three independent biological replicates with at least three technical replicates each (dots) and the error bars represent the standard deviation (SD). Letters above error bars indicate significant differences between samples (α = 0.05, Tukey test). c Concentration range of UmPID14 and UhPID14 peptides was performed. Activity was set to 100% in the absence of inhibitor. Curves were generated using a nonlinear fit and error bars are SEM of three independent replicates

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