Fig. 2 | Nature Communications

Fig. 2

From: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif

Fig. 2

UmPID14 itself is a virulence factor in maize. a Disease rating of maize seedlings at 12 dpi infected with U. maydis SG200 strain, ΔPit2 mutant and complemented ΔPit2 strains with UmPID14 (ΔPit2-UmPID14), UhPID14 (ΔPit2-UhPID14) and UmPit2 (ΔPit2-UmPit2). Number of samples (n). DI: disease index calculated as a mean of at least three biological replicates. Letters above indicate significant differences between samples (α = 0.05, Tukey test). b Disease symptoms of maize seedlings at 12 dpi infected with strains used in (a). c Strategy to generate chimeras for expression in U. maydis SG200 from UmPit2 (yellow) and UhPit2 (green). The signal peptide (SP) from UmPit2 (dark gray) was maintained and the PID14 motif has been exchanged. Gene expression is under control of UmPit2 promotor. d Disease rating of maize seedlings 12 dpi with U. maydis SG200 strain, ΔPit2 mutant and complemented ΔPit2 strain with chimeras UmPit2-UhPID14 (ΔPit2- UmPit2-UhPID14) and UhPit2-UmPID14 (ΔPit2- UhPit2-UmPID14). DI: disease index calculated as a mean of at least three biological replicates. Letters above indicate significant differences between samples (α = 0.05, Tukey test)

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