Fig. 1 | Nature Communications

Fig. 1

From: A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif

Fig. 1

Pit2 homologs cannot complement tumor formation phenotype. a Sequence alignment of Pit2 orthologs from Sporisorium reilianum (Sr), Ustilago maydis (Um), Melanopsichium pennsylvanicum (Mp) and Ustilago hordei (Uh). Signal peptide (gray) and PID14 conserved motif (red) are depicted. Amino acids are labeled depending on their conservation: identical amino acids (asterisk), similar hydrophobicity (colon) or different hydrophobicity (dot). b Disease rating of maize seedlings 12 days post infection (dpi) with U. maydis SG200 solopathogenic strain, SG200 mutant strain lacking Pit2 (ΔPit2) and complemented ΔPit2 strain with U. hordei Pit2 (ΔPit2-UhPit2), S. reilianun Pit2 (ΔPit2-SrPit2) and M. pennsylvanicum Pit2 (ΔPit2-MpPit2). DI: disease index calculated as a mean of at least three biological replicates. Letters above indicate significant differences between samples (α = 0.05, Tukey test). c Disease symptoms of maize seedlings at 12 dpi infected with strains used in (b)