Fig. 4 | Nature Communications

Fig. 4

From: Spatial-fluxomics provides a subcellular-compartmentalized view of reductive glutamine metabolism in cancer cells

Fig. 4

Metabolic rewiring of mitochondrial and cytosolic fluxes under hypoxia. a, b Mass-isotopomer labeling kinetics of citrate in mitochondria (a) and cytosol (b) when feeding HeLa cells with [U-13C]-glutamine under hypoxia. c Mitochondrial to cytosolic metabolite pool size ratios (y-axis) under normoxia (green) and hypoxia (orange) in HeLa cells; average fold change in whole-cell pool size under normoxia and hypoxia is indicated by color (x-axis). d Mitochondrial and cytosolic fluxes in HeLa cells under hypoxia, showing percentages from citrate synthase flux in normoxia. Arrow represents the direction of net flux; number represents net flux in the direction of the arrow and number in parenthesis correspond to the backward flux. Confidence intervals for estimated fluxes shown in Supplementary Data 8. *P < 0.05, **P < 0.01, and ***P < 0.001 by two-sample t-test. Data are mean ± SD, n = 3 independent biological replicates

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