Fig. 2 | Nature Communications

Fig. 2

From: Spatial-fluxomics provides a subcellular-compartmentalized view of reductive glutamine metabolism in cancer cells

Fig. 2

Compartment-specific isotope tracing in mitochondria and cytosol. a A schematic description of the spatial-fluxomics approach. b TCA cycle metabolism and associated reactions involving acetyl-CoA and fatty acid metabolism. c Isotopic labeling kinetics of citrate m + 2 in mitochondria versus cytosol when feeding HeLa cells with [U-13C]-glucose; showing the ratio between the relative abundance of the m + 2 form of citrate in the mitochondria (out of the entire mitochondrial pool) divided by the relative abundance of citrate m + 2 in cytosol. df The isotopic labeling kinetics of citrate m + 4 (d), glutamine m + 5 (e), and glutamate m + 5 (f) when feeding [U-13C]-glutamine. Data are mean ± SD, n = 3 independent biological replicates

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