Fig. 5 | Nature Communications

Fig. 5

From: Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner

Fig. 5

sNPF levels decline with mushroom body-specific attenuation of autophagy and with aging. a Adult brain immunostained for sNPF-precursor. Scale bar: 50 μm. b MB lobes of +/atg5-RNAi and c vt30559/atg5-RNAi immunostained for sNPF precursor (Single Z-plane). Scale bar: 10 μm. d Quantification of signal intensity of sNPF peptide precursor in the MB lobes normalized to control flies (5–6 independent animals; **p < 0.01; Mann–Whitney U-test). e Subesophageal ganglion (SOG) of +/atg5-RNAi and f vt30559/atg5-RNAi immunostained for sNPF precursor (Z-projection). Scale bar: 10 μm. g Quantification of signal intensity of sNPF-peptide precursor in SOG normalized to control flies (n = 6 independent animals; *p < 0.05; Mann–Whitney U-test). MB lobes of (h) 3-day-old w1118 and (j) 30-day-old w1118 immunostained for BRPNc82 (single Z-plane). Scale bar: 10 μm. MB lobes of (i) 3-day-old w1118 and (k) 30-day-old w1118 immunostained for sNPF (Single Z-plane). Scale bar: 10 μm. l Quantification of signal intensity of sNPF peptide precursor in the MB lobes normalized to control flies (9–10 independent animals; ***p < 0.001; Mann–Whitney U-test). SOG of (m) 3-day-old w1118 and (o) 30-day-old w1118 immunostained for BRPNc82 (Single Z-plane). Scale bar: 10 μm. SOG of (n) 3-day-old w1118 and (p) 30 days w1118 immunostained for sNPF (Z-projection). Scale bar: 10 μm. q Quantification of signal intensity of sNPF-peptide precursor in SOG normalized to control flies (9–10 independent animals; ***p < 0.01; Mann–Whitney U-test). In the box and whisker plots, the middle line of a box represents the median (50th percentile) and the terminal lines of a box represent the 25th and 75th percentile. The whiskers represent the lowest and the highest value

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