Fig. 3 | Nature Communications

Fig. 3

From: Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner

Fig. 3

Mushroom body-specific suppression of autophagy induces brain-wide increase of Bruchpilot. Adult brain, 10 days (a) +/atg5-RNAi and (b) elav/atg5-RNAi immunostained for BRPNc82. Scale bar: 50 μm. c Quantification of BRPNc82 intensity within the central brain region normalized to control (n = 11–16 independent brains; ***p < 0.001; Mann–Whitney U-test). Adult brain, 10 days (d) +/atg5-RNAi and (e) ok107/atg5-RNAi immunostained for BRPNc82. Scale bar: 50 μm. f Quantification of BRPNc82 intensity within the central brain region normalized to control (n = 10–16 independent brains; ***p < 0.001; Mann–Whitney U-test). Adult brain, 10 days (g) +/atg9-RNAi and (h) elav/atg9-RNAi immunostained for BRPNc82. Scale bar: 50 μm. i Quantification of BRPNc82 intensity within the central brain region normalized to control (n = 14–15 independent brains; ***p < 0.001; Mann–Whitney U-test). Adult brain, 10 days (j) +/atg9-RNAi and (k) ok107/atg9-RNAi immunostained for BRPNc82. Scale bar: 50 μm. l Quantification of BRPNc82 intensity within the central brain region normalized to control (n = 9–12 independent brains; ***p < 0.001; Mann–Whitney U-test). m MTM of +/atg5-RNAi, +/ok107;mb247-Gal80 and ok107;mb247-Gal80/atg5-RNAi (n = 10–15; nsp > 0.5; One-way ANOVA; Kruskal–Walis post-test). Adult brain, 10 days (n) +/atg5-RNAi, (o) +/ok107;mb247-Gal80, and (p) ok107; mb247-Gal80/atg5-RNAi immunostained for BRPNc82. Scale bar: 50 μm. q Quantification of BRPNc82 intensity within the central brain region normalized to +/atg5-RNAi (n = 5–6 independent brains; nsp > 0.5; One-way ANOVA; Kruskal-Walis test). r STM of +/atg9-RNAi and dilp2/atg9-RNAi (n = 8–9; nsp > 0.5; Mann–Whitney U-test). Adult brain, 10 days (s) atg9-RNAi/+ immunostained for p62/Ref(2)p. Scale bar: 50 μm. Adult brain, 10 days (t) +/atg9-RNAi and (u) dilp2/atg9-RNAi immunostained for BRPNc82. Scale bar: 50 μm. v Quantification of BRPNc82 intensity within the central brain region normalized to control (n = 11–16 independent brains; nsp > 0.5; Mann–Whitney U-test). nsp > 0.01, ***p < 0.0001. In the box and whisker plots, the middle line of a box represents the median (50th percentile) and the terminal lines of a box represent the 25th and 75th percentile. The whiskers represent the lowest and the highest value