Fig. 1 | Nature Communications

Fig. 1

From: Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner

Fig. 1

Neuronal knockdown of atg9 or atg5 suppresses autophagy in the brain. Adult brain, 10 days (a) +/atg9-RNAi, (b) elav/atg9-RNAi, (d) +/atg5-RNAi, and (e) elav/atg5-RNAi immunostained for p62/Ref(2)p. Scale bar: 50 μm. c Quantification of p62/Ref(2)p intensity within the central brain region normalized to control (n = 15–17 independent brains; ***p < 0.001; Mann–Whitney U-test). f Quantification of p62/Ref(2)p intensity within the central brain region normalized to control (n = 23–24 independent brains; ***p < 0.001; Mann–Whitney U-test). g Selective autophagy marker p62/Ref(2)p and autophagosome-associated protein Atg8a accumulate in protein homogenate prepared from the brain of elav/atg9-RNA and elav/atg5-RNAi compared to respective controls. An amount equivalent to 1 brain was loaded onto the gel. Lipidated Atg8a (Atg8a-II) is missing upon KD of atg5 but not when atg9 is knocked down. In the box and whisker plots, the middle line of a box represents the median (50th percentile) and the terminal lines of a box represent the 25th and 75th percentile. The whiskers represent the lowest and the highest value