Fig. 5 | Nature Communications

Fig. 5

From: Distinct G protein-coupled receptor phosphorylation motifs modulate arrestin affinity and activation and global conformation

Fig. 5

Expansions of the series of [15N,1H]TROSY spectra of arrestin-1. It shows representative chemical shift changes in arrestin-1 induced by the 3Pa peptide (ac) and the fully phosphorylated peptide 7P (df, see Supplementary Figure 3 for full spectrum) for E39, L172, and I312 at peptide:arrestin-1 ratios of 0:1 (blue), 1:1 (green), 4:1 (violet), and 10:1 (red). Unassigned resonances are labeled “unass”. A second peak appearing at 10:1 in (a) and (d) which may indicate the presence of multiple states in slow exchange is marked with an asterisk