Fig. 1 | Nature Communications

Fig. 1

From: A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates

Fig. 1

Characterized Hox2 enhancers regulating neural crest (NC) and rhombomeric expression from mouse and lamprey. Schematic diagrams depicting the known enhancers regulating mouse Hoxa2 (a) and Hoxb2 (b), and lamprey hoxα2 (c), in rhombomeres (r) and NC. For each locus, the gene exons are represented by grey boxes and the transcriptional start site by an arrow. Enhancers are marked as black lines below the loci, with their activity domains illustrated by blue shading in schematic dorsal views of the hindbrain (r2–5) and pharyngeal arches (2–3). For the mouse loci, characterised cis-elements contributing to enhancer function are depicted as coloured boxes: hindbrain elements in purple and NC elements in green (not drawn to scale). Known direct inputs from transcription factors into these cis-elements are depicted by arrows, with unknown inputs shown as question marks. Hoxa2 is regulated in r4 and r4-derived NC (PA2) by independent enhancers (a). Hoxb2 expression in r4 and NC is mediated by a single enhancer, through cis-elements bound by Meis and Pbx-Hox factors (b). Since these elements have dual hindbrain/NC activity they are depicted in both purple and green. Genomic regions from the lamprey hoxα2 locus have enhancer activity, with an r2/r4 enhancer positioned within the exons and intron (c). The hoxα2-hoxα3 intergenic region drives reporter expression in the hindbrain and NC. However, it is not known whether this is through independent or shared NC/hindbrain enhancers, specific cis-elements have not been identified, and the relationship of this region to the gnathostome Hoxa2 and Hoxb2 enhancers is unclear

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