Fig. 4 | Nature Communications

Fig. 4

From: Rationally designed carbohydrate-occluded epitopes elicit HIV-1 Env-specific antibodies

Fig. 4

Anti-CONE 1 rabbit sera have moderate inhibitory activity against pseudotyped HIV-1. Pre- and post-immunization sera were heat inactivated, diluted 1:10 in media, and incubated with pseudotyped HIV-1 for 2 h before addition to TZM-bl cell culture. The inducible luciferase activity was quantified as a surrogate of viral infectivity. Percent neutralization (%) was determined by subtracting the pre- and post-immunization relative light units (RLU) from the virus-only RLU (not shown) and dividing the differences by the virus-only RLU. Each experiment was repeated three times. Pseudotyped HIV-1 isolates included in the assays: V682, V1086, and V3037. Rabbits ID: S1201, S1206, S1207 and 481. n = 3 independent experiments. Error bars represent standard deviation. Source data are provided as a Source Data file

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