Fig. 3 | Nature Communications

Fig. 3

From: Rationally designed carbohydrate-occluded epitopes elicit HIV-1 Env-specific antibodies

Fig. 3

Characterization of antibodies in the sera from rabbits immunized with CONEs. a Anti-CONE endpoint titers were determined by ELISA during the immunization process. The data represent the mean titers for each group of rabbits. The boost injections were performed monthly, with bleedings performed three weeks after each boost. n = number of rabbits (4–6, indicated for each immunogen). Error bars represent standard deviation. b Western blots demonstrating the binding of purified rabbit IgG to PNGase-deglycosylated gp120 (~60 kDa band, lanes 2, 4, and 6 in plots of CONE 4 and 5). No interaction between the IgG and glycosylated gp120 (~120 kDa band, lanes 1, 3, and 5 in plots of CONE 4 and 5) was observed. (Left) blots treated with IgG purified from rabbits immunized with CONEs; (Right) same blots stripped from CONE IgG and treated with anti-gp140 IgG as a positive control. Lanes: 1–2, 1086 gp120 D7 293F; lanes 3–4, C.TV1C8.2D11 gp120; lanes 5–6, CHO monomer 1086 D7 gp120 K160N; lane 7, PageRuler Plus Prestained Protein Ladder. Source data are provided as a Source Data file

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