Fig. 5 | Nature Communications

Fig. 5

From: Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons

Fig. 5

Sleep and chromosome dynamics increased following induction of DSBs. a Larvae were treated with etoposide (ETO) during the day (ZT2–4, bd) and night (ZT16–18, eg), and DSBs, chromosome dynamics, and sleep were quantified. b Number of γH2AX foci in single nuclei. During treatment (DMSO: n = 93 cells; ETO: n = 99 cells, *P = 2.2 × 10−8), 1 h following treatment (DMSO: n = 102 cells; ETO: n = 89 cells, *P = 2.2 × 10−6), 2 h following treatment (DMSO: n = 98 cells; ETO: n = 73 cells, *P = 6.8 × 10−4). c Total sleep time. DMSO-treated (n = 84 larvae), ETO-treated (n = 78 larvae), PZT2–3 = 0.18, PZT3–4 = 0.71. Post-treatment: DMSO-treated (n = 84 larvae), ETO-treated (n = 78 larvae), *PZT4–5 = 0.0035, *PZT5–6 = 0.02, PZT6–7 = 0.07, PZT7–8 = 0.2. d Chromosome dynamics in neurons. During treatment (DMSO: n = 22 cells; ETO: n = 11 cells), 1 h following treatment (DMSO: n = 17 cells; ETO: n = 13 cells), 2 h following treatment (DMSO: n = 11 cells; ETO: n = 16 cells, *P = 3.8 × 10−5). Red crosses indicate outliers. eg Number of γH2AX foci in single neuronal nuclei (e, DMSO: n = 118; ETO: n = 138 cells, *P = 1.65 × 10−9), total sleep time (f, DMSO: n = 48; ETO: n = 48 larvae) and chromosome dynamics (g, DMSO: n = 22; ETO: n = 14 cells, *P = 0.02) in DMSO- and ETO-treated larvae during the night. Significant differences of all experiments were determined by two-tailed t-test: two samples assuming unequal variance. Values are presented as boxplots and means (black diamonds) or as dot plots and means ± SEM. Boxplots indicate the median and the 25th-to-75th percentiles. The whiskers extend to the most extreme data points. ZT zeitgeber time. Scale bar = 1 µm

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