Some genetic melanocortin-1 receptor (MC1R) variants responsible for human red hair color (RHC-variants) are consequently associated with increased melanoma risk. Although MC1R signaling is critically dependent on its palmitoylation primarily mediated by the ZDHHC13 protein-acyl transferase, whether increasing MC1R palmitoylation represents a viable therapeutic target to limit melanomagenesis in redheads is unknown. Here we identify a specific and efficient in vivo strategy to induce MC1R palmitoylation for therapeutic benefit. We validate the importance of ZDHHC13 to MC1R signaling in vivo by targeted expression of ZDHHC13 in C57BL/6J-MC1RRHC mice and subsequently inhibit melanomagenesis. By identifying APT2 as the MC1R depalmitoylation enzyme, we are able to demonstrate that administration of the selective APT2 inhibitor ML349 treatment efficiently increases MC1R signaling and represses UVB-induced melanomagenesis in vitro and in vivo. Targeting APT2, therefore, represents a preventive/therapeutic strategy to reduce melanoma risk, especially in individuals with red hair.
Although melanoma accounts only for 1% of skin cancer, it causes the majority of skin cancer-associated deaths. Caucasians in the United States have an approximately 25-fold higher risk of developing melanoma than African Americans, and melanoma risk is almost tripled again in redheads compared to other Caucasians1. The melanocortin-1 receptor (MC1R), a well-known G protein-coupled receptor (GPCR), is the key regulator of hair and skin pigmentation. Upon ultraviolet (UV) irradiation, MC1R is bound by keratinocyte-derived α-melanocyte-stimulating hormone (α-MSH) to activate cAMP signaling, enhance melanin production in melanocytes, and stimulate DNA-damage repair. Human studies and mouse models have demonstrated that MC1R genetic variants are tightly correlated with phenotypes, such as red hair, fair skin, freckling, UV irradiation sensitivity, and melanoma risk. These variants are defined as red-hair-color (RHC) variants2,3. R151C, R160W, and D294H are three most common “strong” red hair variants as they make up >60% of all red hair cases4,5,6,7. R151C and R160W are reported to be associated with red hair, fair skin, and freckles, while D294H only associates with the red hair and freckles phenotype in Caucasians4,5,6,7. These MC1R RHC variants lead to pheomelanin production and make redheads more susceptible to skin cancer8,9.
While many independent studies have demonstrated that melanoma risk is higher in people who carry MC1R RHC variants, the underlying mechanisms are only just being elucidated. The increased melanoma risk attributable to MC1R RHC variants may arise in part through skin pigmentation since pheomelanin in redheads contributes to melanomagenesis through UV radiation (UVR)-independent oxidative damage8,10. However, some MC1R variants are not linked with a red-hair phenotype but remain associated with elevated risk of developing melanoma11,12,13. In Caucasians with melanoma, MC1R variants were detected in 15–33% of dark-haired subjects and 42% of dark-eyed subjects; MC1R variants possibly negate the protective effects of dark pigment. Beyond pigmentation, MC1R plays additional roles in melanoma development. For example, MC1R controls ultraviolet B (UVB)-induced G1-like cell cycle arrest and subsequent onset of premature senescence in melanocytes, abrogation of which contributes to melanoma development14. Moreover, MC1R signaling plays an important role in promoting efficient DNA-damage repair10,15,16,17,18,19,20. Collectively, these observations raise a key question: can therapeutic intervention directed toward enhancing MC1R signaling reverse the increased melanoma risk associated with MC1R RHC variants?
One attractive approach is to increase MC1R palmitoylation, a modification common in GPCRs in which reversible addition of palmitic acid to a cysteine residue of the C-terminal tail or the intracellular loops profoundly affects their structure, stability, membrane localization, or interaction with partner proteins. MC1R palmitoylation is mediated by ZDHHC13 and is essential for activating MC1R signaling9. However, the enzyme(s) required for MC1R depalmitoylation have yet to be identified, though palmitoyl-protein thioesterases (PPTs), including acyl-protein thioesterase-1 (APT1), APT2, and other serine hydrolases21,22,23, represent potential candidates. Importantly, MC1R RHC variants exhibit reduced palmitoylation and consequently defective signaling9. As such, inhibiting MC1R depalmitoylation should enhance signaling from this GPCR and prevent the increased melanoma risk associated with MC1R RHC variants.
Here we report that ZDHHC13 expression correlates with MC1R signaling and survival in human melanoma and that its expression can rescue MC1R RHC variant signaling in vitro and in vivo to suppress UVR-induced melanomagenesis. Significantly, we reveal that MC1R de-palmitoylation is catalyzed by APT2 and consequently ML349, an APT2 inhibitor, rescues defects in MC1R RHC variant signaling and offers a potential avenue to an effective melanoma prevention strategy.
Clinical relevance of ZDHHC13 and MC1R signaling in human melanomas
Although the protein-acyl transferase ZDHHC13 catalyzes MC1R palmitoylation to activate MC1R signaling9, the clinical significance of this observation is not known. By using RNA sequencing (RNA-seq) data from the human The Cancer Genome Atlas (TCGA) melanoma cohort and GEPIA (Gene Expression Profiling Interactive Analysis)24, we found that the mRNA levels of ZDHHC13 positively correlated with those of well-known targets downstream from MC1R signaling (Fig. 1a, b), including MITF and DCT25. MITF is the master regulator of melanocyte development and regulates multiple cellular processes, including promoting survival, differentiation and proliferation, and suppressing senescence and melanoma invasion26,27,28,29,30. In addition, we used TCGA clinical data and GEPIA24 and found that high mRNA levels of ZDHHC13 are associated with a survival benefit in melanoma patients (Fig. 1c). These results suggest that higher expression levels of ZDHHC13 is correlated with stronger activation of MC1R signaling in human melanomas.
Transgenic ZDHHC13 increases MC1RR151C signaling in vivo
The role of ZDHHC13 as the key protein s-acyl transferase for MC1R was previously identified in vitro9. However, whether it plays a similar role in regulating MC1R and its downstream signaling in pigment production and melanomagenesis in vivo remain unclear. To address this issue, we developed a transgenic mouse with melanocyte-specific ZDHHC13 expression controlled by the mouse Tyr enhancer/promoter31 using the same strategy used by us previously to generate the MC1R RHC variant mice (C57BL/6J-MC1Re/eJ/MC1RR151C-Tg, as R151C is the most common “strong” red hair allele in humans4,5,6,7) (Supplementary Fig. 1a). The ZDHHC13 expression construct was injected into single-cell embryos of C57BL/6J mice and the resulting transgenic ZDHHC13 was genotyped by PCR (Supplementary Fig. 1b). We noted that Tg-ZDHHC13 mice exhibited higher skin eumelanin/pheomelanin ratios than control mice (Fig. 2a, b). We next selected a Tg-ZDHHC13 mouse with medium levels of exogenous ZDHHC13 expression to cross with our previously generated MC1R RHC variant mice and palmitoylation-defective MC1R mutant C315S mice9 to generate Tg-ZDHHC13/MC1R RHC variant mice. The offspring that express the MC1RR151C variant show a mosaic coat color, whereas those that express MC1RC315S exhibit red hair phenotype similar to MC1Re/e mice9. Tg-ZDHHC13/MC1RR151C mice exhibited a darker coat color than Tg-MC1RR151C mice (Fig. 2a), with uniform pigmentation in individual hairs (Supplementary Fig. 1c). However, no obvious fur/skin color change was observed in MC1RWT or transgenic expressing the non-palmitoylatable MC1RC315S mutant, with or without transgenic ZDHHC13 (Fig. 2a and Supplementary Fig. 1d)9. These results suggest that exogenous ZDHHC13 overexpression rescues eumelanin production in MC1R RHC variant mice in vivo.
To further test the in vivo effect of ZDHHC13 on MC1R RHC variants, we collected mouse ears and tails, where epidermal melanocytes are located32, and evaluated eumelanin production. Enhanced pigmentation was observed in the ears and tails of the Tg-ZDHHC13/MC1RR151C mice compared to MC1RR151C alone (Fig. 2c, d), a result confirmed by Fontana–Masson staining (Fig. 2e and Supplementary Fig. 1e) and biochemical assays (Fig. 2f). Notably, Tg-ZDHHC13 expression did not enhance eumelanin production in MC1RWT and MC1RC315S mice (Fig. 2c–f and Supplementary Fig. 1d, e). As the number of melanocytes in the hair follicle in Tg-MC1R-variants and Tg-ZDHHC13 was not altered compared to the non-Tg-ZDHHC13 controls (Supplementary Fig. 1f–j), the increased eumelanin production observed did not arise owing to ZDHHC13-stimulation of melanocyte proliferation.
To identify whether transgenic ZDHHC13 activated MC1R palmitoylation in vivo, we used the acyl-biotin exchange (ABE) palmitoylation assay to detect palmitoylated MC1R protein in mouse primary melanocytes (MPM) isolated from 3.5-day-old Tg-ZDHHC13/MC1RR151C mice. MC1R proteins extracted from the isolated MPMs were pulled down with specific anti-MC1R antibodies and then treated sequentially with N-ethylmaliemide and hydroxylamine to expose palmitoylated cysteines. Streptavidin was used to detect biotin-BMCC-labeled MC1R palmitoylation protein. We found that Tg-ZDHHC13 slightly increased MC1R protein palmitoylation in MC1RWT but remarkably enhanced MC1R protein in Tg-MC1RR151C mice (Fig. 2g). These results suggest that ZDHHC13 overexpression physiologically rescues MC1R RHC-induced “red hair” phenotype by increasing MC1R palmitoylation in a mouse model.
Inhibition of UVB-induced melanomagenesis in redheads in vivo
To investigate the potential role for ZDHHC13 in tumor formation, Tyr-Cre-BRAFCA mice (B6.Cg-Braftm1Mmcm Tg(Tyr-cre/ERT2)Bos/BosJ)33 were crossed with the Tg-ZDHHC13/MC1RR151C mice to get Tyr-Cre-BRAFCA/Tg-ZDHHC13/MC1RR151C mice. Tyr-Cre-ERT2 fusion gene31 activity was then induced by tamoxifen administration to activate BRAFV600E expression specifically in melanocytes. Mice were then given 500 J/m2 UVB irradiation, the minimal erythema dose (MED) of UVB in mice9,34,35, once each week for 4 weeks and then observed for melanoma incidence for a further 90 days (Fig. 3a). Melanomas were observed in the UVB-exposed Tyr-Cre-BRAFCA/Tg-ZDHHC13/MC1RR151C mice, with the Tyr-Cre-BRAFCA/Tg-ZDHHC13 mice and Tyr-Cre-BRAFCA/Tg-MC1RR151C mice serving as controls. Melanoma was first diagnosed 24, 39, and 57 days after the final UV irradiation in mice expressing the palmitoylation defective MC1R (MC1RC315S), MC1R RHC variant (MC1RR151C), and MC1RWT respectively, indicating that melanoma incidence was associated with MC1R status. Ninety days after the final UVR treatment, melanoma was diagnosed in 92% (11/12), 64% (9/14), and 19% (3/16) of mice with MC1RC315S, MC1RR151C, and MC1RWT, respectively (Fig. 3b). More importantly, our data indicated that melanoma incidence was much lower and with delayed first diagnosis (day 50 compared to day 39) in the Tg-ZDHHC13/MC1RR151C mice than in the MC1RR151C mice (27% (4/15) vs. 64% (9/14), log-rank test, p = 0.0457; Fig. 3b). All melanomas diagnosed in these variant mice displayed similar morphological and histologic features (Fig. 3c and Supplementary Fig. 2a). Previous report showed that UVR accelerates BRAFV600E-induced melanomagenesis by inducing UVR-signature C>T mutations at mouse Trp53, including H39Y, S124F (homolog to human TP53 S127), R245C (homolog to human TP53 R248), and R270C (homolog to human TP53 R273)36. S127, R248, and R273 are all human melanoma TP53 mutation hotspots. To test the mutations in our model, we sequenced PCR fragments covering these Trp53 mutation spots. Mutations in Trp53 (S124F, R245C, or R270C) were detected in 4/4 Tyr-Cre-BrafV600E-MC1RR151C melanomas but only 1/4 (R270C) in Tyr-Cre-BrafV600E-MC1RR151C-ZDHHC13 mice melanomas (Supplementary Fig. 2b). Our results therefore show that ZDHHC13-activated MC1R palmitoylation plays a critical role in melanoma prevention in vivo.
Inhibition of APT2 inhibits UVB-induced melanomagenesis in vivo
The results so far present a potential therapeutic anti-melanoma strategy. Palmitoylation is a dynamic and reversible process controlled both by palmitoyl acyltransferases, which mediate palmitoylation, and PPTs, which remove this modification (Fig. 4a and Supplementary Fig. 3a). Increasing MC1R palmitoylation in vivo would potentially diminish melanoma incidence, especially for redheads in whom MC1R signaling is compromised. To increase MC1R palmitoylation, we compared the effects of three commercially available depalmitoylation inhibitors, including Palmostatin B (Palm-B)23, ML348, and ML34937. Palm-B is a newly described inhibitor of the deacylating enzymes APT1, APT2, and ABHD17 and therefore specifically inhibits depalmitoylation38, whereas ML348 is a newly described selective APT1 inhibitor and ML349 is a selective APT2 inhibitor37.
We first compared the effect of increasing concentrations (10, 100, 500, 1000 nM) of Palm-B, ML348, or ML349 on cAMP levels in human primary melanocytes (HPMs) engineered to express MC1RR151C together with α-MSH (1 µM). We found that ML349 was the most efficient at increasing cAMP production in melanocytes expressing the MC1RR151C variant (Fig. 4b). These results suggest that ML349 potentially activates palmitoylation of MC1R and that targeted inhibition of APT2 inhibition might be a strategy to prevent melanomagenesis in redheads.
To confirm that APT2 affects MC1R palmitoylation, HPMs co-infected with HA-APT1/Flag-MC1R or HA-APT2/Flag-MC1R-expressing viruses were assessed for MC1R protein palmitoylation using the ABE assay. We found that expression of APT2, but not APT1, decreased MC1R palmitoylation and that knockdown of APT2 upregulated MC1R palmitoylation (Supplementary Fig. 3b–d). We also found using co-immunoprecipitation that MC1R specifically interacted with APT2, but not APT1 (Supplementary Fig. 3e–h). Notably, in melanocytes knockdown of APT2 stimulated palmitoylation of the MC1RR151C RHC variant but not the palmitoylation-defective MC1RC315S mutant (Supplementary Fig. 3i–j). Collectively, these data strongly suggest that APT2 is the key MC1R depalmitoylation enzyme.
We next asked whether APT2-regulated MC1R palmitoylation contributes to the control of UVR-induced premature senescence in melanocytes9,14. APT2 was ectopically expressed or knocked down in HPMs and the resulting cells irradiated with a low dose of UVB (25 J/m2), before assaying for senescence associated β-gal activity. We found that overexpression of APT2 augmented low-dose UVB-induced premature senescence in melanocytes engineered to express either the MC1R RHC variant or wild-type (WT) MC1R (Supplementary Fig. 4a). By contrast, knockdown of APT2 inhibited UVB-induced and MC1R RHC-augmented melanocyte premature senescence (Supplementary Fig. 4a).
Having established a key role for APT2 in regulation of MC1R RHC variant function in vivo, we next evaluated the role of the specific APT2-targeted inhibitor, ML34937, in activating MC1R signaling and inhibiting melanomagenesis. To this end, HPMs engineered to express stably short hairpin RNA (shRNA) targeting endogenous MC1R and re-expressing MC1RR151C or WT MC1R were pretreated with ML349 (100 nM) and α-MSH (1 µM) for 30 min following 100 J/m2 UVB treatment. MC1R protein palmitoylation was then detected using the ABE palmitoylation assay 3 h after UV radiation. The dose of 100 J/m2 is the standard erythema dose (SED) of UVB, and the ambient exposure over an entire sunny summer day in Europe (Switzerland) is approximately 30–40 SED39. While the MC1RC315S mutant was not palmitoylated as expected, ML349 inhibited depalmitoylation of both the WT MC1R and MC1RR151C proteins (Fig. 4c). Stimulation of the same engineered HPMs with ML349 (100 nM) and α-MSH (1 µM) revealed that ML349 induced cAMP production in melanocytes expressing the RHC MC1RR151C variant (Fig. 4d). These results confirm that ML349 is potentially an effective therapeutic agent that can rescue the defects in MC1R RHC variant cAMP signaling.
Using genetically engineered human immortal melanocytes (hTERT/p53DD/CDK4(R24C))9,14,40 and melanoma xenograft mouse models, we examined the potential for APT2 inhibition by ML349 to impact melanocyte malignant transformation in vitro and ex vivo. To this end, we compared the effect of the three depalmitoylation inhibitors ML349, ML348, and Palm-B on hTERT/p53DD/CDK4(R24C) melanocytes in which expression of endogenous MC1R was suppressed using shRNA and MC1RR151C was reintroduced together with BRAFV600E. Colony-formation and soft agar assays were performed to identify malignant transformation. We found that treatment with ML349 inhibited the MC1RR151C augmented BRAFV600E-mediated malignant transformation more efficiently than Palm-B or ML348 (Supplementary Fig. 4b, c). Similar results were obtained using soft agar assays (Supplementary Fig. 4d, e) and were further confirmed using mouse xenograft studies in which ML349 treatment markedly inhibited MC1RR151C-augmented BRAFV600E-mediated malignant transformation (Fig. 4e–g and Supplementary Fig. 4f).
The preventative effect of ML349 on melanomagenesis in redheads was validated using our previously generated MC1R RHC variant mice (Tyr-Cre-BRAFCA/C57BL/6J-MC1Re/eJ/MC1RR151C-Tg) that were given a dose of 500 J/m2 UVB irradiation each week for 4 weeks and then observed for melanoma incidence for another 90 days. In the UVB radiation period, 5 mg/kg ML349 was injected intraperitoneally into mice prior to the treatment with UV. While melanoma was first diagnosed 37 days after the final UV irradiation in mice without treatment, ML349 delayed the onset of melanoma to 56 days. Furthermore, 90 days after the final UVR, melanoma was diagnosed in 23% (3/13) or 64% (7/11) of mice with or without ML349 treatment, respectively (p = 0.0366; Fig. 4h). In addition, mutations of Trp53 hotspots (R245C or R270C)36 were detected in 3/4 Tyr-Cre-BrafV600E-MC1RR151C melanomas but not in Tyr-Cre-BrafV600E-MC1RR151C+ML349 mice melanomas (Supplementary Fig. 4g). As no strong side effects of ML349 treatment were observed, these data suggest that ML349 is a potential small molecule to inhibit melanomagenesis in redheads.
The activation of α-MSH/MC1R signaling in melanocytes requires palmitoylation9 and is engaged in three important pathways for melanoma development: pigment production41,42, DNA repair15,16,17,18,19, and phosphatase and tensin homolog signaling14. Protein palmitoylation is controlled by the balance between protein-acyl transferases and the depalmitoylating enzymes (palmitoyl protein thioesterases) that remove this modification. Although several protein-acyl transferases are expressed in melanocytes, previous work9 identified ZDHHC13 as the major MC1R palmitoylation enzyme. Indeed, ZDHHC13 overexpression increased the palmitoylation of MC1R variants to prevent melanocyte malignant transformation in vitro and ex vivo9.
There are two Tyr-CreERT2-BRAFCA melanoma mouse models: the “Marais–Larue”43 and the “McMahon–Bosenberg”33 models. Both of them are conditional BRAFV600E knock in mouse models, but the “Marais–Larue” homozygous BRAFV600E mice are lethal during early development. Moreover, they used two different Tyr-CreERT2 mice that have different characteristics31,44. For instance, Tyr-CreERT2 mice published by Bosenberg and colleagues31 transcribe CreERT2 in melanocyte stem cells, but Tyr-CreERT2 published by Yajima and colleagues44 does not transcribe CreERT2 in melanocyte stem cells. Our preliminary observations were substantiated here using our newly generated transgenic ZDHHC13 mice to demonstrate that MC1R palmitoylation and downstream signaling can be activated by ZDHHC13 in vivo to suppress melanomagenesis in “McMahon–Bosenberg” model, with 500 J/m2 UVB irradiation per week for 4 weeks.
Although we have focused here on ZDHHC13 in melanocyte biology and MC1R signaling in particular, it seems likely that this crucial enzyme may have a broader role as a protector of skin integrity; previous studies have demonstrated that mice with a homozygous mutation in ZDHHC13, which results in a stop codon upstream of the DHHC domain, exhibit phenotypes with severe hair and skin defects45.
Although ZDHHC13 was identified as the primary protein-acyl transferase mediating MC1R palmitoylation, the critical MC1R depalmitoylating enzyme had not previously been identified. Two depalmitoylation enzymes, ATP1 and ATP2, are known to control protein depalmitoylation21,46,47. Although the two enzymes are very similar, some studies have suggested that APT1 and APT2 may not be functionally redundant and might exhibit substrate specificity. For example, APT2, not APT1, promotes GAP-43 depalmitoylation48, whereas APT1, but not APT2, promotes β2-adrenergic receptor depalmitoylation49. Similarly, it has been shown that Scribble S-palmitoylation is regulated by APT2, but not APT150. In addition, the high-resolution structure study of APT2/ML349 and APT1/ML348 complex reveal different conformations in the active sites further suggested that APT1 and APT2 may have unique roles and different functions46. Here our biochemical studies characterized APT2 as the major depalmitoylating enzyme of MC1R in melanocytes. Our data are therefore consistent with the two enzymes possessing distinct target specificities and indicate that APT2, but not APT1, catalyzes MC1R depalmitoylation. APT1 might not act as a depalmitoylating enzyme of MC1R because it is predominantly localized in mitochondria51, whereas MC1R is a plasma membrane-localized GPCR. Significantly, we demonstrate that ML349, a specific APT2 inhibitor, effectively enhances MC1R palmitoylation, increases downstream signaling, and reverses the deleterious effects of the RHC MC1RR151C variant.
The potential ability to modulate MC1R function for clinical benefit, especially in redheads, has long been an ambition in skin cancer biology, though to date an effective strategy has been elusive. For example, the cyclic AMP agonist forskolin can increase pigmentation and subsequently prevent melanomagenesis25. Unfortunately, topical administration is problematic since forskolin does not readily penetrate the epidermis providing a major barrier for its translation to clinical use. The identification here of ZDHHC13 as a critical regulator of MC1R function in vivo offers an alternative and potentially viable approach toward melanoma prevention in highly susceptible individuals. Since palmitoylation is dynamic and reversible9,52,53,54, we can envisage either increasing ZDHHC13 function or decreasing the activity of the MC1R depalmitoylation enzyme that we identify here as APT2. Since depalmitoylation inhibitors are already available, we consider inhibition of APT2 as the most promising approach.
Although the depalmitoylation inhibitor Palm-B can activate MC1R signaling9 and is effective against both APT1 and APT2, the two major protein-depalmitoylating enzymes55, it also increases palmitoylation of HRAS and NRAS23. While Palm-B does not affect cell viability, it does induce loss of steady-state localization of RAS proteins and phenotypic reversion of HRASG12V-transformed MDCK-F3 cells23. However, recent activity-based protein profiling studies found that Palm-B not only inhibits APT1 and APT2 but also inactivates many other serine hydrolases, including ABHD6, ABHD16A, ABHD17A-C, PNPLA6, and FASN38. Thus, although Palm-B remains a popular tool to study protein palmitoylation, its limited specificity means that it is unlikely to be used clinically. On the other hand, the recent development of more selective APT inhibitors, including ML348 (selective APT1 inhibitor, Ki = 280 nM) and ML349 (selective APT2 inhibitor, Ki = 120 nM)37, offered a potential opportunity to increase the activity of MC1R RHC variants by increasing their palmitoylation. Our studies here indicate that targeting APT2 to rescue MC1R palmitoylation using ML349, rather than using the generic depalmitoylation inhibitor PALM-B, may represent an effective strategy for melanoma prevention, especially in redheads.
UVB treatment of cells
All UVB exposure was performed as previously described9. Briefly, cells were washed by phosphate-buffered saline (PBS) and exposed to UVB in the Stratalinker UV chamber (Stratagene, Cedar Creek, TX, USA), in which the emittance of UVB was measured by the UV photometer (UVP LLC, Upland, CA, USA). HPMs were isolated from normal discarded foreskins as previously described9. Isolated HPMs were cultured in Medium 254 with melanocyte supplement (Thermo Fisher Scientific Inc., Waltham, MA, USA). All cell lines used are mycoplasma negative and were authenticated.
Mouse experiment protocols were provided by the Institutional Animal Care and Use Committee (IACUC) of Boston University School of Medicine. All mice were maintained in pathogen-free facility of Animal Science Center of Boston University Medical Campus and strictly followed the instructions by IACUC of Boston University School of Medicine. Mice were housed on a time cycle of 12 h of light and 12 h of dark and allowed free access to sterilized diet and water. The mice were monitored daily for health and distress status. For the tumorigenesis assay of engineered hTERT/p53DD/CDK4(R24C)/BRAFV600E melanocytes14,40, 3 × 106 cells were 1:1 mixed with matrigel and injected subcutaneously into the flanks of NCr nude mice (8–10 weeks, Taconic Biosciences). Tumor size was measured by caliper and did not exceed 2 cm at the largest diameter. Transgenic mice were generated as previously described9 by Boston University School of Medicine Transgenic and Genome Engineering Core and Mouse ES Cell & Transgenic Facility, Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology (MIT). The expression construct was designed such that the targeted gene was inserted downstream of murine tyr promoter/enhancer region. The transgene was detected by genotyping mice tail or ear DNA using the primers targeting fragment across tyr promoter/enhancer and ZDHHC13 (GGGCTATGTACAAACTCCAAGA, CAGCTTCCAAAAGCTTATCAACT). C57BL/6J-MC1Rvariant-Tg mice were generated as previous described9. Tyr-Cre-BRAFCA mice (B6.Cg-Braftm1Mmcm Tg(Tyr-cre/ERT2)Bos/BosJ) were purchased from The Jackson Laboratory, Stock No: 017837. Mice collected for melanomagenesis assay were administered with tamoxifen (T5648) (Sigma-Aldrich, St. Louis, MO, USA) in corn oil daily by intraperitoneal injection of 0.12 mg/g for a week. The control mice received corn oil injection. Mice UVB treatment procedure was performed as described previously9. The mice were irradiated 1 day per week for 500 J/m2 UVB treatment, which is equivalent to the mouse MEDs of UVB and commonly used as a measurement for sunlight in vivo. Specifically, mice were treated with UVB in a custom-made lucite chamber (Plastic Design Corporation, MA, USA) for 12 min. Mice were allowed freedom of movement during UVB treatment. A double bank of UVB lamps were used and UVA was filtered by the chamber. The UV emittance was measured by UV photometer (UV Products, Upland, CA, USA).
Isolation of mouse primary melanocytes
Mouse primary melanocytes were isolated from the dorsolateral skin of 3.5-day postnatal mice. The skin was washed by PBS and then digested in 0.25% trypsin overnight. The epidermal layer was separated and minced following a centrifugation at 1000 × g for 10 min. Lastly, the cells were cultured in cell culture incubator with 10% CO2 in Medium 254 with melanocyte supplement (Thermo Fisher Scientific Inc., Waltham, MA, USA) for 14 days.
Plasmids and infection
All MC1R plasmids were generated as previously described9,14. To generate the expression plasmids for retroviral infection, the cDNAs were subcloned into pQCXIP (Clonetech) at the NotI/EcoRI sites, respectively. Mutants were generated by the QuickChange II Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA). To knockdown MC1R or APT2 in HPMs, shRNA in pLKO.1 targeting MC1R or APT2 were co-transfected with psPAX2 and pMD2.G in HEK293 (ATCC) using Lipofectamine 3000 (Thermo Fisher Scientific Inc., Waltham, MA, USA). Lentiviruses were collected after 48 h and then used to infect cells for 24 h in the presence of polybrene (8 μg/mL) and the infected cells were selected with puromycin (2 μg/mL). To generate cells with expression of MC1R variants or APT2, HEK293 cells were co-transfected with MC1R variants or APT2 in pQCXIP, VSV-G, and pUMVC plasmids using Lipofectamine 3000 (Thermo Fisher Scientific Inc., Waltham, MA, USA). Retroviruses were collected after 48 h and cells were infected with retroviruses in the presence of polybrene (8 μg/mL). After 24 h, cells were selected with puromycin (2 μg/mL). shRNA constructs targeting human MC1R (RHS4533-EG4157) and human APT2 (RHS4533-EG11313) were purchased from Open Biosystems (GE Healthcare Dharmacon, Inc., Lafayette, CO, USA). The most efficient knockdown of shMC1R (AAATGTCTCTTTAGGAGCCTG) and shAPT2 (AAGAAATTCCTTCACAGCTGC) were used in experiment.
Western blot and immunoassay
Western blot, immunoprecipitation, and the ABE palmitoylation assay were performed as previously described9,14. Lysis buffer contains 50 mM Tris pH 7.4, 1% Triton X-100, 0.5 mM EDTA, 0.5 mM EGTA, 150 mM NaCl, 10% glycerol, 1 mM phenylmethanesulfonylfluoride, and protease inhibitor cocktail (Pierce, Thermo Fisher Scientific Inc., Waltham, MA, USA). The supernatant was collected after centrifugation at 15,000 × g for 15 min at 4 °C, and cell lysate was precleared by 20 µL Protein G Agarose Beads (Thermo Fisher Scientific Inc., Waltham, MA, USA) and then incubated with primary antibodies overnight at 4 °C or Flag/HA beads (Sigma-Aldrich) for 2 h. Streptavidin-HRP (1:2000) (21130) were purchased from Thermo Fisher Scientific Inc. Anti-ZDHHC13 (1:1000) (ab28759), anti-MC1R (1:1000) (ab125031), and anti-APT2 antibodies (1:500) (ab151578, ab87231) were purchased from Abcam. Anti-MC1R antibody (N-19) (sc-6875) (1:500) was purchased from Santa Cruz Biotechnologies, Inc. Anti-β-actin−peroxidase antibody (AC15) (1:20000), anti-Flag-peroxidase antibody (1:2000) (A8592), anti-HA−peroxidase antibody (H6533) (1:1000), anti-Flag agarose beads (A2220), anti-HA agarose beads (A2095), anti-mouse secondary antibody (A4416) (1:2000), and anti-rabbit secondary antibody (A-4914) (1:2000) were purchased from Sigma-Aldrich. All western blots shown are representatives of three independent experiments. Most important uncropped blot are shown in Supplementary Fig. 5. cAMP levels were measured by the cAMP Direct Immunoassay Kit (ab65355) (Abcam, Cambridge, MA, USA) following Abcam’s protocol, and optical density at 450 nm were measured.
Immunohistochemistry and tumor analysis
Immunohistochemistry was performed as previously described9,34,56. Briefly, 10% formalin solution was used to fix mouse melanomas at 4 °C for overnight. Then the samples were paraffin-embedded and cut into 5-μm-thick sections at Boston University School of Medicine core facility. For antigen retrieval, sections were heated in a boiling water bath for 20 min in 10 mM pH 6.0 sodium citrate buffer. TBS with 0.1% Tween-20 and 5% normal goat serum was used to block the sections. Then tissue sections were incubated with anti-S-100 (1:200) (Dako North America, Inc. Carpinteria, CA, USA) primary antibody at 4 °C overnight and subsequently incubated with secondary antibody and DAB substrate (DAB/Metal Concentrate and Stable Peroxide Substrate Buffer, Thermo Fisher Scientific Inc., Waltham, MA, USA). Lastly, coverslips were mounted by Permount (Thermo Fisher Scientific Inc., Waltham, MA, USA). For mutation measurement, genomic DNA was extracted from paraffin-embedded mouse melanoma sections by using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany). PCR products covering selected mutations were sent directly for DNA sequencing as described36.
The whole back skin of mice was collected for eumelanin/pheomelanin measurement. Eumelanin and pheomelanin were quantified by high-performance liquid chromatography based on the level of pyrrole-2,3,5-tricarboxylic acid (PTCA) by alkaline hydrogen peroxide oxidation of eumelanin and 4-amino-3-hydroxyphenylalanine (4-AHP) by hydriodic acid reductive hydrolysis of pheomelanin, respectively. Final results were determined by a conversion as described (eumelanin = 45 × PTCA, pheomelanin = 9 × 4-AHP)57. All results are calculated by three independent experiments.
Clonogenic survival and soft agar assay
The clonogenic survival and soft agar assay were performed as previously described14,40. Briefly, engineered hTERT/p53DD/CDK4(R24C)/BRAFV600E melanocytes were exposed to 20 J/m2 UVB with pretreatment of 1 μM α-MSH before plating into 6-well plate at 1000 cells per well. Fifteen days after UVB treatment, crystal violet was used to stain colonies. For soft agar assay, 10,000 cells were seeded in 0.5% low-melting-point agarose in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), layered onto 0.8% agarose in DMEM with 10% FBS. After 30 days, the colonies >50 μm were counted under a light microscope. All results are calculated by three independent experiments.
Pearson’s correlation between ZDHHC13 and MITF or DCT in TCGA SKCM and melanoma patient survival analysis based on ZDHHC13 expression (cutoff = 50%) were calculated by GEPIA (http://gepia.cancer-pku.cn/)24.
Student’s t test was performed for all quantitative data between different groups, and the statistical significance was labeled as *p < 0.05, **p < 0.01, ***p < 0.001. All quantitative data were presented as the mean ± s.d. or ± s.e.m as labeled of at least three independent experiments.
Further information on experimental design is available in the Nature Research Reporting Summary linked to this article.
All data that support the findings of this study are available on reasonable request to the corresponding authors. The contributing authors declare that all relevant data are included in the paper and its supplementary information files.
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R.C. is supported by National Institutes of Health (NIH), U.S. Department of Health & Human Services. J.Z. is supported by the National Key R&D Program of China (2017YFA0503502) and the National Natural Science Foundation of China (31730050). C.R.G. is funded by the Ludwig Institute for Cancer Research.
The authors declare no competing interests.
Journal peer review information: Nature Communications thanks Lionel Larue, Viktor Umansky, and the other anonymous reviewer(s) for their contribution to the peer review of this work.
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