Fig. 2 | Nature Communications

Fig. 2

From: Non-lytic clearance of influenza B virus from infected cells preserves epithelial barrier function

Fig. 2

Epithelial cells are infected by IBV and survive in the large airways of mice. a Flow cytometry of mNeon− (total cells) and mNeon+ (infected cells) from the lungs of Mal/04-mNeon infected mice at 2 DPI, stained for EpCam (epithelial lineage) and CD45 (hematopoietic lineage). b Quantification of total and mNeon+ infected cell expression of EpCam and CD45. n = 3 mice per sample, two-way ANOVA. c Flow cytometry of tdTomato− (total cells) and tdTomato+ (survivor cells) from the lungs of Mal/04-Cre infected tdTomato Cre reporter transgenic mice at 14 DPI, stained for EpCam (epithelial lineage) and CD45 (hematopoietic lineage). d Quantification of total and tdTomato+ survivor cell expression of EpCam and CD45. n = 3 mice per sample, two-way ANOVA. e Flow cytometry of epithelial (EpCam+ ) tdTomato+ survivor cells from Mal/04 WT and Mal/04-Cre infected mouse lungs. f Quantification of tdTomato+ cells from murine lungs. n = 5 mice per sample, Student’s T-test. g Microscopy of 14 DPI Mock and Mal/04-Cre infected murine lungs with subpanels showing airways (bronchiole and trachea) and alveolar region. Scale bar = 100 μm, inset scale bar = 50 μm. S.E.M. was plotted to indicate variability around the mean for tested samples, *p ≤ 0.05, **p ≤ 0.001, ns = not significant

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