Fig. 1: | Nature Communications

Fig. 1

From: A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Fig. 1

MOVIE detects native transcripts at the site of transcription in living zebrafish embryos. a Left: schematic of the detection principle of transcript imaging by MOVIE at the miR430 primary miRNA gene cluster. Right: Image of whole embryo, imaged in vivo by lightsheet microscopy, injected with three antisense, FITC-labelled morpholinos (MO) targeting miR430 priRNA (green) and mRuby3:H2B fusion protein as nuclear marker (red). Arrows point at signal accumulation, appearing as double green punctae within the nuclei, labelled by mRuby3:H2B (arrowheads). b Representative images of live embryos, imaged in vivo at high/oblong by lightsheet microscopy, injected at zygote stage with triple and single sense, antisense and mismatch-containing, FITC-labelled morpholinos and transcription blockers (α-amanitin or triptolide, n = 6 embryos each). c Transcript signal detection before and after 1-hour triptolide treatment started at 256-cell stage (0 h), (n = 6 embryos each). Stages of embryos as indicated in panels. trip triptolide, as antisense, s sense, MO morpholino oligonucleotide (where number depicts specific MO, for details see Supplementary Table 1), 5 MM 5 mismatch containing, h hours after treatment. Scale bars: a 50 µm b, c 10 µm