Fig. 4 | Nature Communications

Fig. 4

From: Cyanobacterial antimetabolite 7-deoxy-sedoheptulose blocks the shikimate pathway to inhibit the growth of prototrophic organisms

Fig. 4

7dSh (1) prevents regeneration of resuscitating Synechocystis. a Optical density (black) and PSII quantum yield (turquoise) of chlorotic Synechocystis cultures (initial OD750 = 0.5) regenerating in the absence or presence of 7dSh. NaNO3 (17.3 mM) and 7dSh (ca. 260 µM) were added in aqueous solution at 0 h. Values represent the mean values of three biological replicates; standard deviations are indicated. Dots indicate data distribution. b Oxygen evolution of resuscitating Synechocystis cultures (initial OD750 = 0.5) upon addition of nitrate (NaNO3, 17.3 mM) in the presence or absence of 7dSh (ca. 206 µM). Significant differences between 7dSh treatment and untreated control for each timepoint were analyzed in an unpaired t-test (*p-value < 0.05; **p-value < 0.01; *** p-value < 0.001; n.s., not significant). Values represent the mean values of three biological replicates; standard deviations are indicated. Dots indicate data distribution. c Cultures of chlorotic Synechocystis (initial OD750  = 0.5) 48 h after addition of nitrate (NaNO3, 17.3 mM) and 7dSh. Numbers indicate concentration (µg mL−1) of 7dSh added to the culture. Source data are provided as a Source Data file

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