Fig. 8 | Nature Communications

Fig. 8

From: Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes

Fig. 8

GITR is expressed on human ILC2s and augments Th2 cytokine production. FACS-sorted human blood ILC2s were cultured with recombinant human (rh) IL-2, rhIL-7, and rhIL-33 and plate bound GITR-L-Fc or isotype control, human IgG, n = 6. a Representative FACS plots of h-GITR expression after 0, 24, and 48 h of stimulation on ILC2s and corresponding quantitation presented as MFI. b Representative FACS plot of h-GITR staining on VAT-ILC2s isolated from healthy subjects and corresponding quantitation, n = 4. c The levels of IL-5, IL-13, GM-CSF, IL-8, and IL-9 in the culture supernatants were measured by Luminex after 24 h of stimulation. Data representative of 6 individual blood donors, n = 6. Error bars are the mean ± SEM. Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001

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