Fig. 7 | Nature Communications

Fig. 7

From: Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes

Fig. 7

GITR engagement induces NF-κB pathway signaling in ILC2s. a Volcano plot comparison of whole transcriptome gene expression of sorted WT ILC2s from VAT treated with isotype control or DTA-1 (5 µg/mL) for 24 h in vitro, n = 3. Differentially expressed genes (defined as statistically significant adjusted p-value < 0.05) with changes of at least 1.5 fold-change (FC) are shown in red. Notable differentially expressed genes are labeled. b Heat plot of differentially expressed genes. c Selected genes plotted as the normalized counts in isotype control-treated compared to DTA-1-treated mice. Differentially expressed genes (defined as in a) are shown in red. Gray area indicates region of 1.5 fold-change cutoff or lower change in expression. Shown are expression of cytokine and cytokine receptor genes. d Upregulated (red) and downregulated (green) genes in the NF-κB pathway. e Representative histogram of the expression of NF-κB p65 in isolated ILC2s from mice challenged with IL-33 and cultured in vitro for 24 h with DTA-1 (red) or isotype control (black). The level of isotype-matched stain control is shown as a gray-filled histogram. f Corresponding quantification presented as Mean Fluorescence Intensity of NF-κB p65 with DTA-1 or isotype control. g Network analysis of upregulated (red) and downregulated (green) genes overall significantly predicted to inhibit the apoptosis of leukocytes. h Representative flow cytometry plots of VAT Lin-CD45+IL-7R+ST2+Annexin V+ 7-AAD+ ILC2s from C57/BL6 or GITR-/- mice (n = 5) and corresponding quantitation presented as the percentage of ILC2s. Error bars are the mean ± SEM. Student’s t-test, *p < 0.05, ***p < 0.001

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