Fig. 4 | Nature Communications

Fig. 4

From: Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes

Fig. 4

GITR engagement drives ILC2-derived secretion and M2 macrophage polarization. A cohort of Rag2-/- mice were fed on HFD and either treated with DTA-1 (1 mg/mouse) or isotype control by intraperitoneal injection every four days, n = 8. a The frequency of CD45+ cells of the VAT stromal vascular fraction (SVF) was quantified by flow cytometry. b Representative flow cytometry plots of VAT Lin-CD45+IL-7R+ST2+ ILC2s in each group after 14 weeks of treatment, and corresponding quantitation presented as the number of ILC2s per gram of VAT. c Representative flow cytometry plots of intracellular IL-5 (top panel) and IL-13 (bottom panel) in VAT ILC2s and corresponding quantitation after 14 weeks of treatment, presented as frequency of positive ILC2s. d Gating strategy of macrophages in mouse VAT as CD45+ CD11bhi F4/80hi. e Quantification of the number of CD45+ CD11bhi F4/80hi macrophages per gram of VAT. f Representative flow cytometry plots of CD45+CD11bhiF4/80hiCD206+CD11c+ M2 macrophages in VAT and corresponding quantitation after 14 weeks of treatment. Error bars are the mean ± SEM. Student’s t-test, *p < 0.05, ***p < 0.001

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