Fig. 3 | Nature Communications

Fig. 3

From: Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes

Fig. 3

Preventive GITR engagement protects from Type 2 Diabetes in Rag2-/- mice. a A cohort of Rag2-/- mice were fed on HFD and either treated with DTA-1 (1 mg/mouse) or isotype control by intraperitoneal injection every four days according to the scheme, n = 8. b Total weight and c blood glucose levels were measured every two weeks for 14 weeks. d Plasma insulin concentration was measured by ELISA. e Glucose tolerance test and f insulin tolerance test were performed in a cohort of mice after 14 weeks of treatment. The corresponding area under the curve was calculated for each group. g Hematoxylin and eosin-stained epididymal adipose tissue sections (x400). Red arrows point to recruited inflammatory cells around adipocytes, scale bars, 100 µm. h Corresponding quantitation presented as the mean adipocyte area. i Percentage of fat mass was measured using a body composition analyzer. j, k CLAMS analysis was performed using individually housed groups of DTA-1 or isotype control treated Rag2-/- mice maintained on a HFD. j Variations in the absolute oxygen consumption and k energy expenditure normalized by total body weight were measured. l Uncoupling protein 1 (UCP1) immunohistochemistry (IHC) in epididymal adipose tissue sections (x400), scale bars, 100 µm after 14 weeks of treatment and m Ucp1 transcripts levels in VAT lysates, n = 8. Error bars are the mean ± SEM. Student’s t-test, *p < 0.05, **p < 0.01. Mouse image provided with permission from Servier Medical Art

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