Fig. 3 | Nature Communications

Fig. 3

From: The binding of Borealin to microtubules underlies a tension independent kinetochore-microtubule error correction pathway

Fig. 3

CPC-microtubule interaction is important for maintenance of taxol dependent spindle assembly checkpoint arrest. a Schematic of experimental procedure for bd. b Representative time-lapse phase-contrast images, from one of two independent replicates, of a cell treated as in a. Arrow head points to the cell that enters mitosis and is arrested in presence of 100 nM taxol and exits mitosis, time of mitotic entry and exit are depicted. Duration of mitotic arrest is the duration between mitotic entry and exit. c Box and whisker graph of duration of mitosis in cells expressing the indicated Borealin transgene (n = 255 for BorealinWT, n = 261 for BorealinMTBM, n = 209 for BorealinΔ20, and n = 110 for PRC1MBD-BorealinΔ20, combined data from two independent repeats). d Graph of data from c showing cumulative frequency distribution of duration of mitosis for the cells expressing indicated Borealin transgene. e Box and whisker graph of duration of mitosis in presence of 100 nM taxol. Cells were treated as in a with the exception that 1 μg/ml of doxycycline was added, at the time of second thymidine addition, for induction of CenpBDBD-INCENP747-918 expression (n = 109 for BorealinWT and CenpBDBD-INCENP747-918 BorealinWT, n = 110 for BorealinMTBM, and n = 106 for CenpBDBD-INCENP747-918 BorealinMTBM, combined data from two independent repeats). Statistics performed using Dunn's Multiple Comparison Test, ***P < 0.001; **P < 0.01, *P < 0.05 and ns P > 0.05. All Box and whisker plots represent the median (central line), 25th–75th percentile (bounds of the box) and 5th–95th percentile (whiskers). Scale bar is 5 μm

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