Fig. 2 | Nature Communications

Fig. 2

From: High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Fig. 2

Fourier decomposition of cell shape. a First six components of radial Fourier function (shape modes) including component zero, red dots indicate the origin. b Shape mode analysis applied to dynamic measurements of HL60 cells arranged for even and odd coefficients. Each blue trace represents the amplitude of a Fourier component of a single cell over the axial position z in the channel (n = 1580 cells). The yellow lines indicate the mean over all traces whereas the red vertical lines visualize the inlet and outlet position. c Reconstruction of cell deformation from first four even (left panel) and first five odd (right panel) Fourier coefficients and a0 with mean cell shapes at channel inlet and outlet (top). d Reconstruction of cell deformation from first ten Fourier coefficients, including mean cell shapes (top). Inset shows the relative error in circularity of the reconstructed traces relative to the original dataset. Measurements have been carried out in a 30 × 30 μm channel at a flow rate of 8 nl s−1

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